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首页> 外文期刊>Analytical chemistry >Simultaneous Identification and Antimicrobial Susceptibility Testing of Multiple Uropathogens on a Microfluidic Chip with Paper-Supported Cell Culture Arrays
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Simultaneous Identification and Antimicrobial Susceptibility Testing of Multiple Uropathogens on a Microfluidic Chip with Paper-Supported Cell Culture Arrays

机译：纸支持细胞培养芯片在微流控芯片上同时鉴定和多种致病菌的药敏试验

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摘要

A microfluidic chip was developed for one-step identification polydimethylsiloxane (PDMS) microchip used had features of cell culture he chamber arrays connected through a sample introduction channel. At the bottom of each chamber, a paper substrate preloaded with chromogenic media and antimicrobial agents was embedded. By integrating a hydrophobic membrane valve on the microchip, the urine sample can be equally distributed into and confined in individual chambers. The identification and AST assays on multiple uropathogens were performed by combining the spatial resolution of the cell culture arrays and the color resolution from the chromogenic reaction. The composite microbial testing assay was based on dynamic changes in color in a serial of chambers. The bacterial antimicrobial susceptibility was determined by the lowest concentration of an antimicrobial agent that is capable of inhibiting the chromogenic reaction. Using three common uropathogenic bacteria as test models, the developed microfluidic approach was demonstrated to be able to complete the multiple colorimetric assays in 15 h. The accuracy of the microchip method, in comparison with that of the conventional approach, showed a coincidence of 94.1%. Our data suggest this microfluidic approach will be a promising tool for simple and fast uropathogen testing in resource-limited settings.
机译：开发了一种微流控芯片，用于一步鉴定聚二甲基硅氧烷（PDMS）微芯片，该芯片具有通过样品导入通道连接的细胞培养室阵列的功能。在每个腔室的底部，嵌入预装有发色介质和抗菌剂的纸质基底。通过在微芯片上集成疏水膜阀，可以将尿液样本均匀分配并限制在各个腔室中。通过结合细胞培养阵列的空间分辨率和发色反应的颜色分辨率，对多种尿路病原进行鉴定和AST分析。复合微生物测试方法基于一系列小室中颜色的动态变化。细菌的抗菌药敏性由能够抑制发色反应的抗菌剂的最低浓度确定。使用三种常见的尿路致病细菌作为测试模型，已证明开发的微流控方法能够在15小时内完成多种比色测定。与常规方法相比，微芯片方法的准确性显示出94.1％的重合。我们的数据表明，这种微流体方法将是在资源有限的环境中进行简单快速的尿路致病菌检测的有前途的工具。

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