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首页> 外文期刊>Analytica chimica acta >Coupling continuous ultrasound-assisted extraction with ultrasonic probe, solid-phase extraction and high-performance liquid chromatography for the determination of sodium Danshensu and four tanshinones in Salvia miltiorrhiza bunge
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Coupling continuous ultrasound-assisted extraction with ultrasonic probe, solid-phase extraction and high-performance liquid chromatography for the determination of sodium Danshensu and four tanshinones in Salvia miltiorrhiza bunge

机译：连续超声辅助萃取与超声探针耦合，固相萃取和高效液相色谱法测定丹参中丹参素和四种丹参酮的含量

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A dynamic continuous ultrasound-assisted extraction with high intensity ultrasonic probe (CUAE-HIUP) combined with solid-phase extraction (SPE) for preconcentration and clean-up of the extract prior to high-performance liquid chromatography (HPLC) determination of the main biological active ingredients, sodium Danshensu and four tanshinones (dihydrotanshione 1, tanshinone 1, cryptotanshinone and tanshinone IIA) from root of Salvia miltiorrhiza bunge has been developed. An experimental design (Plackett-Burman design, 2(6) x 3/16) was used to optimize the CUAE-HIUP procedure and the SPE step. Detection limits of 1.2-4.2 x 10(-6) mg mL(-1) were achieved with the preconcentration efficiency of more than 10-folds by the SPE procedure. The repeatability and within-laboratory reproducibility of the whole process, expressed as relative standard deviations (RSDs), were 1.5-3.6%, 1.6-3.1%, respectively. As compared with the conventional extraction techniques such as room temperature extraction (24 h), Soxhlet (4 h) and even microwave-assisted extraction (2 min), CUAE-HIUP achieved the highest extraction yield (98.9%) and the least amount (10 mL) of solvent compared with the other techniques (16 mL) in only 3 min. The method was successfully applied to the determination of the five biological active ingredients in root of S. miltiorrhiza bunge and Danshen-containing pharmaceutical formulations. (c) 2007 Elsevier B.V. All rights reserved.
机译：动态连续超声辅助萃取与高强度超声探针（CUAE-HIUP）结合固相萃取（SPE）用于预浓缩和净化萃取物，然后进行高效液相色谱（HPLC）测定主要生物已开发出丹参根中的活性成分丹参素钠和四种丹参酮（二氢丹参酮1，丹参酮1，隐丹参酮和丹参酮IIA）。实验设计（Plackett-Burman设计，2（6）x 3/16）用于优化CUAE-HIUP程序和SPE步骤。通过SPE程序实现的检出限为1.2-4.2 x 10（-6）mg mL（-1），预浓缩效率超过10倍。整个过程的可重复性和实验室内可重复性，以相对标准偏差（RSD）表示，分别为1.5-3.6％，1.6-3.1％。与常规提取技术（如室温提取（24小时），索氏提取法（4小时）甚至微波辅助提取（2分钟）相比，CUAE-HIUP的提取率最高（98.9％），而提取量最少（仅在3分钟内与其他技术（16 mL）的溶剂相比（10 mL）。该方法已成功地用于测定丹参和含丹参药物制剂根中的五种生物活性成分。 （c）2007 Elsevier B.V.保留所有权利。

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