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首页> 外文期刊>Analytica chimica acta >Use of CdSe/ZnS luminescent quantum dots incorporated within sol-gel matrix for urea detection
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Use of CdSe/ZnS luminescent quantum dots incorporated within sol-gel matrix for urea detection

机译：溶胶-凝胶基质中掺入的CdSe / ZnS发光量子点用于尿素检测

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摘要

In this work,urea detection techniques based on the pH sensitivity of CdSe/ZnS QDs were developed using three types of sol-gel membranes:a QD-entrapped membrane,urease-immobilized membrane and double layer consisting of a QD-entrapped membrane and urease-immobilized membrane.The surface morphology of the sol-gel membranes deposited on the wells in a 24-well microtiter plate was investigated.The linear detection range of urea was in the range of 0-10 mM with the three types of sol-gel membranes.The urea detection technique based on the double layer consisting of the QD-entrapped membrane and urease-immobilized membrane resulted in the highest sensitivity to urea due to the Michaelis-Menten kinetic parameters.That is,the Michaelis-Menten constant(K_m=2.0745 mM)of the free urease in the QD-entrapped membrane was about 4-fold higher than that(K_m=0.549 mM)of the immobilized urease in the urease-immobilized membrane and about 12-fold higher than that(K_m=0.1698 mM)of the immobilized urease in the double layer.The good stability of the three sol-gel membranes for urea sensing over 2 months showed that the use of sol-gel membranes immobilized with QDs or an enzyme is suitable for biomedical and environmental applications.
机译：在这项工作中，使用三种类型的溶胶-凝胶膜开发了基于CdSe / ZnS QD的pH敏感性的尿素检测技术：夹带QD的膜，固定有脲酶的膜以及由夹有QD的膜和脲酶组成的双层研究了24孔微量滴定板中孔上沉积的溶胶-凝胶膜的表面形态。三种类型的溶胶-凝胶的线性检测范围为0-10 mM。由于具有Michaelis-Menten动力学参数，基于QD捕获膜和固定有脲酶固定膜的双层结构的尿素检测技术对尿素的灵敏度最高，即Michaelis-Menten常数（K_m =夹带QD的膜中的游离脲酶2.0745 mM约为固定化脲酶的膜中固定脲酶的（K_m = 0.549 mM）约4倍，比（K_m = 0.1698 mM）高约12倍固定化脲酶三种溶胶-凝胶膜对尿素感测的良好稳定性超过2个月，表明使用固定有QDs或酶的溶胶-凝胶膜适合生物医学和环境应用。

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