...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Analytica chimica acta >Mass spectrometry signal amplification for ultrasensitive glycoprotein detection using gold nanoparticle as mass tag combined with boronic acid based isolation strategy
【24h】

Mass spectrometry signal amplification for ultrasensitive glycoprotein detection using gold nanoparticle as mass tag combined with boronic acid based isolation strategy

机译：质谱信号放大用于金纳米颗粒作为质量标签结合基于硼酸的分离策略进行超灵敏糖蛋白检测

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We describe a novel method for rapid and ultrasensitive detection of intact glycoproteins without enzymatic pretreatment which was commonly used in proteomic research. This method is based on using gold nanoparticle (AuNP) as signal tag in laser desorption/ionization mass spectrometry (LDI-MS) analysis combined with boronic acid assisted isolation strategy. Briefly speaking, target glycoproteins were firstly isolated from sample solution with boronic acid functionalized magnetic micrqpartides, and then the surface modified gold nanoparticles were added to covalently bind to the glycoproteins. After that, these AuNP tagged glycoproteins were eluted from magnetic micropartides and applied to LDI-MS analysis. The mass signal of AuNP rather than that of glycoprotein was detected and recorded in this strategy. Through data processing of different standard glycoproteins, we have demonstrated that the signal of AuNP could be used to quantitatively represent glycoprotein. This method allows femtomolar detection of intact glycoproteins. We believe that the successful validation of this method on three different kinds of glycoproteins suggests the potential use for tracking trace amount of target glycoproteins in real biological samples in the near future.
机译：我们描述了一种无需酶预处理即可快速和超灵敏地检测完整糖蛋白的新方法，该方法通常用于蛋白质组学研究。该方法基于结合硼酸辅助分离策略的金纳米颗粒（AuNP）作为激光解吸/电离质谱（LDI-MS）分析中的信号标签。简而言之，首先用硼酸官能化的磁性微颗粒从样品溶液中分离目标糖蛋白，然后添加表面修饰的金纳米颗粒以共价结合糖蛋白。之后，将这些AuNP标签的糖蛋白从磁性微粒中洗脱出来，并用于LDI-MS分析。在该策略中检测并记录了AuNP的质量信号而不是糖蛋白的质量信号。通过对不同标准糖蛋白的数据处理，我们证明了AuNP信号可用于定量表示糖蛋白。该方法可以完成完整糖蛋白的飞摩尔检测。我们相信，在三种不同类型的糖蛋白上成功验证该方法表明，在不久的将来可能会用于追踪真实生物样品中痕量的目标糖蛋白。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号