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首页> 外文期刊>Analytica chimica acta >Ultrasensitive enzyme-free immunoassay for squamous cell carcinoma antigen using carbon supported Pd-Au as electrocatalytic labels Jian Gao, Bin Du, Xiaoyue Zhang, Aiping Guo, Yong Zhang, Dan Wu, Hongmin Ma,
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Ultrasensitive enzyme-free immunoassay for squamous cell carcinoma antigen using carbon supported Pd-Au as electrocatalytic labels Jian Gao, Bin Du, Xiaoyue Zhang, Aiping Guo, Yong Zhang, Dan Wu, Hongmin Ma,

机译：Ultrasensitive enzyme-free immunoassay for squamous cell carcinoma antigen using carbon supported Pd-Au as electrocatalytic labels Jian Gao, Bin Du, Xiaoyue Zhang, Aiping Guo, Yong Zhang, Dan Wu, Hongmin Ma,

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摘要

A novel nonenzymatic sandwich-type electrochemical immunosensor has been developed to detect squamous cell carcinoma antigen (SCCA). Nitrogen-doped graphene sheet (N-GS) was used to increase capacity of capturing primary antibodies (Ab1). Carbon-supported Pd-Au binary nanoparticles (Pd-Au/C) were synthesized and used to label secondary antibodies (Ab2). The specific binding of SCCA and antibodies enabled a quantitative attachment of Pd-Au/C on the electrode surface. Electrocatalytic analysis showed that the prepared Pd-Au/C exhibit excellent electrocatalytic activity towards hydrogen peroxide (H2O2). We use current response of electrocatalytic labels Pd-Au/C to detect the concentration of SCCA. The unique nonenzymatic immunosensor exhibits a relatively wide linear range from 0.005 to 2 ng mL~(-1) and high sensitivity with a low detection limit of 1.7 pg mL~(-1). The immunsensor also shows good reproducibility (4.2%) and stability (5.8%), which makes it an enormous application prospect in clinical research.
机译：已经开发了一种新型的非酶夹心型电化学免疫传感器来检测鳞状细胞癌抗原（SCCA）。氮掺杂石墨烯片（N-GS）用于增加捕获一抗（Ab1）的能力。合成了碳载Pd-Au二元纳米颗粒（Pd-Au / C），并用于标记二抗（Ab2）。 SCCA和抗体的特异性结合使得Pd-Au / C可以定量附着在电极表面。电催化分析表明，所制得的Pd-Au / C对过氧化氢（H2O2）表现出优异的电催化活性。我们使用电催化标记Pd-Au / C的电流响应来检测SCCA的浓度。独特的非酶免疫传感器具有从0.005到2 ng mL〜（-1）的相对较宽的线性范围，并具有高灵敏度和低检测限1.7 pg mL〜（-1）。该免疫传感器还具有良好的重现性（4.2％）和稳定性（5.8％），使其在临床研究中具有广阔的应用前景。

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