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首页> 外文期刊>Analytical and bioanalytical chemistry >Detection of influenza virus by a biosensor based on the method combining electrochemiluminescence on binary SAMs modified Au electrode with an immunoliposome encapsulating Ru (II) complex
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Detection of influenza virus by a biosensor based on the method combining electrochemiluminescence on binary SAMs modified Au electrode with an immunoliposome encapsulating Ru (II) complex

机译：基于结合二元SAMs修饰的Au电极上的化学发光与包封Ru（II）复合物的免疫脂质体的方法的生物传感器检测流感病毒

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摘要

Recently, point of care testing (POCT) used for diagnosis of influenza infection has a problem showing false negative diagnosis because of the low sensitivity. We would like to report detection of influenza virus A (H1N1) by an immunosensor based on electrochemiluminescence (ECL) that uses an immunoliposome encapsulating tris(2,2'-bipyridyl)ruthenium(II) complex. By using the sensor, we could detect the virus that competed with hemagglutinin (HA) peptide immobilized on self-assembled monolayers (SAMs) in immunoreaction of the antibody bound on the surface of liposome. The HA peptide was 19 mer (TGLRNGITNKVNSVIEKAA). We demonstrated great improvement of sensitivity and accuracy by introducing binary SAMs instead of mono SAMs. The binary SAMs was prepared from 3,3'-dithiodipropionic acid and 1-hexanethiol. Use of the binary SAMs enabled to increase the SAMs coverage on Au electrode; the fact was confirmed by observation of the cathodic desorption currents. By using such an electrode, first the detection method of BSA was optimized to lower ECL background signal. Then we applied the method to the detection of influenza virus. We could successfully detect the virus with higher sensitivity compared with that by POCT and ELISA. The detection range was from a concentration of 2.7x 10(2) to 2.7 x 10(3) PFU/mL.
机译：近来，由于敏感性低，用于诊断流感感染的即时检验（POCT）存在显示假阴性诊断的问题。我们想报告一种基于电化学发光（ECL）的免疫传感器对流感病毒A（H1N1）的检测，该电化学传感器使用包裹了三（2,2'-联吡啶基）钌（II）复合物的免疫脂质体。通过使用该传感器，我们可以检测与固定在自组装单层（SAMs）上的血凝素（HA）肽竞争的脂质体表面抗体发生免疫反应的病毒。 HA肽为19聚体（TGLRNGITNKVNSVIEKAA）。通过引入二进制SAM而不是单SAM，我们证明了灵敏度和准确性的极大提高。由3，3'-二硫代二丙酸和1-己硫醇制备二元SAM。使用二进制SAM可以增加SAM在金电极上的覆盖范围;通过观察阴极解吸电流证实了这一事实。通过使用这种电极，首先优化了BSA的检测方法，以降低ECL背景信号。然后我们将该方法应用于流感病毒的检测。与POCT和ELISA相比，我们可以成功地检测出具有更高灵敏度的病毒。检测范围是从2.7 x 10（2）到2.7 x 10（3）PFU / mL的浓度。

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