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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Modulation of the connexin26 tumor suppressor gene expression through methylation in human mammary epithelial cell lines.
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Modulation of the connexin26 tumor suppressor gene expression through methylation in human mammary epithelial cell lines.

机译：通过甲基化在人乳腺上皮细胞系中调节连接蛋白26抑癌基因的表达。

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摘要

BACKGROUND: Normal mammary epithelial cells express mainly gap junction connexin 26 (Cx26) that is either reduced or absent in breast cancers. Since connexin gene mutations are rare we examined if Cx26 gene repression is related to hypermethylation. MATERIALS AND METHODS: Five breast epithelial cell lines were examined for Cx26 mRNA expression and hypermethylation. Treatment with a DNA methyltransferase inhibitor, 5-Aza-2'-deoxycytidine (5-Aza-CdR), was carried out to determine if Cx26 gene expression could be upregulated. RESULTS: Cx26 expression was easily detectable in an immortalized human mammary epithelial cell line (MCF-10) and markedly diminished (MDA-MB231) or undetectable in (MCF-7, BT-20, T47-D) breast cancer cell lines. Hypermethylation of the Cx26 5' region was observed in MCF-10 and MCF-7 cells. Treatment with 5-Aza-CdR resulted in slight or no induction in Cx26 expression in breast cancer cell lines. CONCLUSIONS: Hypermethylation is unlikely to be a major mechanism for Cx26 gene repression in human mammary cancer cell lines.
机译：背景：正常的乳腺上皮细胞主要表达间隙连接蛋白26（Cx26），在乳腺癌中这种连接蛋白减少或不存在。由于连接蛋白基因突变很少见，因此我们检查了Cx26基因抑制是否与甲基化过度有关。材料与方法：检查了五个乳腺上皮细胞系的Cx26 mRNA表达和甲基化程度。用DNA甲基转移酶抑制剂5-Aza-2'-脱氧胞苷（5-Aza-CdR）进行处理，以确定Cx26基因表达是否可以上调。结果：在永生化的人类乳腺上皮细胞系（MCF-10）中很容易检测到Cx26表达，而在（MCF-7，BT-20，T47-D）乳腺癌细胞系中则明显检测不到Cx26表达。在MCF-10和MCF-7细胞中观察到Cx26 5'区域的超甲基化。用5-Aza-CdR处理导致乳腺癌细胞系中Cx26表达的轻微或不诱导。结论：甲基化不太可能是抑制人类乳腺癌细胞Cx26基因的主要机制。

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