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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >A Biomimicking Tumor Tissue Model Using Hepatocellular Carcinoma Cell Sheet in a Collagen Sandwich System
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A Biomimicking Tumor Tissue Model Using Hepatocellular Carcinoma Cell Sheet in a Collagen Sandwich System

机译：在胶原蛋白三明治系统中使用肝细胞癌细胞片的仿生肿瘤组织模型

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摘要

Cytotoxic efficacy of anticancer drugs has been widely studied with monolayer-cultured cancer cells. However, the drug efficacy under two-dimensional (2D) culture conditions usually differs from that of three-dimensional (3D) culture conditions. In the present study, an in vitro tumor tissue model was constructed by sandwiching a cancer cell monolayer (cancer cell sheet) between two collagen layers as a biomimicking tumor tissue model, and the in vitro cytotoxic efficacy of doxorubicin was investigated. In the biomimicking tumor tissue model, hypoxic areas were observed, and the secretion of vascular endothelial growth factor increased time-dependently. Additionally, cell growth inside the model was significantly slower than that of the 2D culture system. The in vitro cytotoxicity of doxorubicin with the 3D system decreased compared to the 2D system, suggesting that the surrounding collagen layer acted as a barrier much like cancer stroma does. Consequently, this study successfully demonstrated that the cancer cell sheet in collagen sandwich configuration provides a useful in vitro tumor tissue model.
机译：对于单层培养的癌细胞，抗癌药的细胞毒性功效已得到广泛研究。但是，在二维（2D）培养条件下的药物功效通常不同于三维（3D）培养条件。在本研究中，通过将癌细胞单层（癌细胞片）夹在两个胶原蛋白层之间作为生物模拟肿瘤组织模型来构建体外肿瘤组织模型，并研究了阿霉素的体外细胞毒性作用。在仿生肿瘤组织模型中，观察到缺氧区域，并且血管内皮生长因子的分泌随时间增加。此外，模型内部的细胞生长明显比2D培养系统慢。与2D系统相比，使用3D系统的阿霉素的体外细胞毒性降低了，这表明周围的胶原蛋白层就像癌症基质一样起着屏障的作用。因此，这项研究成功地证明了胶原三明治结构的癌细胞薄片提供了有用的体外肿瘤组织模型。

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