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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Anticancer Effects of Brominated Indole Alkaloid Eudistomin H from Marine Ascidian Eudistoma viride Against Cervical Cancer Cells (HeLa)
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Anticancer Effects of Brominated Indole Alkaloid Eudistomin H from Marine Ascidian Eudistoma viride Against Cervical Cancer Cells (HeLa)

机译：海洋生物海藻中溴化吲哚生物碱大戟属H对宫颈癌细胞（HeLa）的抗癌作用。

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摘要

Marine invertebrates called ascidians are prolific producers of bioactive substances. The ascidian Eudistoma viride, distributed along the Southeast coast of India, was investigated for its in vitro cytotoxic activity against human cervical carcinoma (HeLa) cells by the MTT assay. The crude methanolic extract of E. viride, with an IC50 of 53 mu g/ml, was dose-dependently cytotoxic. It was more potent at 100 mu g/ml than cyclohexamide (1 mu g/ml), reducing cell viability to 9.2%. Among nine fractions separated by chromatography, ECF-8 exhibited prominent cytoxic activity at 10 mu g/ml. The HPLC fraction EHF-21 of ECF-8 was remarkably dose- and time-dependently cytotoxic, with 39.8% viable cells at 1 mu g/ml compared to 51% in cyclohexamide-treated cells at the same concentration; the IC50 was 0.49,mu g/ml. Hoechst staining of HeLa cells treated with EHF-2I at 0.5 mu g/ml revealed apoptotic events such an cell shrinkage, membrane blebbing, chromatin condensation and formation of apoptotic bodies. Cell size and granularity study showed changes in light scatter, indicating the characteristic feature of cells dying by apoptosis. The cell-cycle analysis of HeLa cells treated with fraction EHF-21 at 1 mu g/ml showed the marked arrest of cells in G(0)/G(1), S and G(2)/M phases and an increase in the sub G(0)/G(1) population indicated an increase in the apoptotic cell population. The statistical analysis of the sub-G(1) region showed a dose-dependent induction of apoptosis. DNA fragmentation was also observed in HeLa cells treated with EHF-21. The active EHF-2I fraction, a brominated indole alkaloid Eudistomin H, led to apoptotic death of HeLa cells.
机译：被称为海生动物的海洋无脊椎动物是生物活性物质的多产者。通过MTT分析研究了分布在印度东南沿海的海鞘Eudistoma viride对人宫颈癌（HeLa）细胞的体外细胞毒活性。大肠杆菌的粗制甲醇提取物的IC50为53μg / ml，具有剂量依赖性的细胞毒性。它在100μg / ml下比环己酰胺（1μg / ml）更有效，将细胞活力降低到9.2％。在通过色谱分离的九个馏分中，ECF-8以10μg / ml的浓度显示出显着的细胞毒活性。 ECF-8的HPLC馏分EHF-21具有明显的剂量和时间依赖性，具有1μg/ ml的39.8％活细胞，而在相同浓度的环己酰胺处理的细胞中有51％。 IC50为0.49μg/ ml。用0.5μg / ml EHF-2I处理的HeLa细胞的Hoechst染色显示凋亡事件，例如细胞萎缩，膜起泡，染色质浓缩和凋亡小体形成。细胞大小和粒度研究表明光散射发生变化，表明细胞因凋亡而死亡的特征。用1μg / ml的EHF-21馏分处理的HeLa细胞的细胞周期分析显示，细胞在G（0）/ G（1），S和G（2）/ M期显着停滞，并且在亚G（0）/ G（1）群体表明凋亡细胞群体增加。 sub-G（1）地区的统计分析表明凋亡的剂量依赖性诱导。在用EHF-21处理的HeLa细胞中也观察到DNA断裂。活性EHF-2I馏分（一种溴化的吲哚生物碱Eudistomin H）导致HeLa细胞凋亡性死亡。

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