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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Active Estrogen Synthesis and its Function in Prostate Cancer-derived Stromal Cells
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Active Estrogen Synthesis and its Function in Prostate Cancer-derived Stromal Cells

机译：活性雌激素合成及其在前列腺癌衍生基质细胞中的功能

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Background: It remains unclear whether estrogen is produced in prostate cancer (PCa) and how it functions in PCa. Materials and Methods: To examine the production of estrogen in PCa cells, the concentration of estrogen in the medium in which LNCaP cells and PCa-derived stromal cells (PCaSC) were co-cultured, was measured by liquid chromatography-mass spectrometry/mass spectrometry (LCMS/MS), while aromatase (CYP19) mRNA expression was confirmed by real-time polymerase chain reaction (RT-PCR) methods. To verify whether estrogen is synthesized from testosterone in PCaSC functions, PCaSC were co-cultured with breast cancer MCF-7-E10 cells, which were stably-transfected with ERE-GFP, in the presence of testosterone. GFP expression was detected when PCaSCs could synthesize estrogen. The proliferation of PC-3 cells in the presence of PCaSC was determined by cell count. Results: PCaSC metabolized excessive testosterone to estrogen, which activated estrogen receptor in breast cancer cells. Moreover, estrogen synthesized from testosterone in PCaSC regulated the proliferation of PC-3 cell via repression of some unknown growth factors that were secreted from PCaSC. Conclusion: A chimeric co-culture method between breast cancer cells and PCaSC revealed the production of active estrogen in PCaSC. High-dose testosterone therapy might introduce a new potential strategy to treat CRPC.
机译：背景：目前尚不清楚前列腺癌（PCa）中是否产生雌激素，以及它在PCa中如何发挥作用。材料与方法：为了检测PCa细胞中雌激素的产生，通过液相色谱-质谱/质谱法测量了LNCaP细胞和PCaSC基质细胞（PCaSC）共培养的培养基中雌激素的浓度。 （LCMS / MS），而通过实时聚合酶链反应（RT-PCR）方法确认了芳香化酶（CYP19）mRNA的表达。为了验证是否在PCaSC功能中由睾丸激素合成了雌激素，将PCaSC与乳腺癌MCF-7-E10细胞共培养，并在睾丸激素存在下用ERE-GFP稳定转染了它们。 PCaSCs可以合成雌激素时检测到GFP表达。通过细胞计数确定在PCaSC存在下PC-3细胞的增殖。结果：PCaSC将过多的睾丸激素代谢为雌激素，从而激活了乳腺癌细胞中的雌激素受体。此外，在PCaSC中由睾丸激素合成的雌激素通过抑制PCaSC分泌的一些未知生长因子来调节PC-3细胞的增殖。结论：乳腺癌细胞与PCaSC之间的嵌合共培养方法揭示了PCaSC中活性雌激素的产生。大剂量睾丸激素治疗可能会引入治疗CRPC的新潜在策略。

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