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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >MicroRNA Expressions in HPV-induced Cervical Dysplasia and Cancer
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MicroRNA Expressions in HPV-induced Cervical Dysplasia and Cancer

机译：HPV诱导的宫颈发育异常和癌症中的MicroRNA表达。

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Background/Aim: The role of oncogenic or high-risk human papillomavirus (HPV) in cervical carcinogenesis is inevitable, yet not fully understood. Detailed analysis of microRNA (miRNA) alterations occurring during high-risk HPV transformation will increase our current understanding over cervical carcinogenesis. The two main aims of the study were: (i) finding association between HPV infection characteristics and socio-demographic variables, (ii) finding an predictors of clinical outcome. Materials and Methods: The expression levels of different microRNAs (miR-21, miR-27a, miR-34a, miR-155, miR-196a, miR-203) were determined in formalin-fixed paraffin-embedded (FFPE) human HPV-positive cervical dysplastic and tumorous tissue samples using quantitative real-time PCR (qPCR). Sociodemographic and life-style factors were also analyzed. Results: The expression of miR-27a was significantly higher in cervical intraepithelial neoplasia (CIN)2-3 compared to CIN1 (p=0.023) and in squamous cell carcinoma (SCC) compared to CIN2-3 (p=0.033). Moreover, significantly lower levels of miR-34a were detected in CIN2-3 than in CIN1 (p=0.041) and in SCC than in CIN2-3 (p=0.021). Furthermore, we found significant differences in subjects with multiple HPV in miR-27a (p=0.015) and miR-203 (p=0.025) in CIN2-3 compared to CIN1 and miR-21 (p=0.002), mir-27a (p=0.001) and miR-34a (p=0.001) in SCC/CIN2-3. Expression of miR-27a, showing up-regulation in CIN2-3 compared to CIN1 (p=0.028) and miR-34a (down-regulated), correlated with HPV 16 positivity (CIN2-3/CIN1: p=0.027 and SCC/CIN2-3: p=0.036). MiR-34a expression was also significantly altered in connection to smoking status and presence of HPV 16. Conclusion: The demand for additional, alternative molecular biomarkers with prognostic potential is strong. Evaluation of miRNA expression might be helpful to distinguish different cervical lesions and might be able to help in the prediction of HPV infection outcome.
机译：背景/目的：致癌或高风险的人类乳头瘤病毒（HPV）在宫颈癌发生中的作用是不可避免的，但尚未完全了解。对高危HPV转化过程中发生的微小RNA（miRNA）改变的详细分析将增加我们目前对宫颈癌发生的了解。该研究的两个主要目的是：（i）在HPV感染特征和社会人口统计学变量之间找到关联，（ii）寻找临床结果的预测指标。材料和方法：在福尔马林固定石蜡包埋的（FFPE）人HPV-RNA中测定不同microRNA（miR-21，miR-27a，miR-34a，miR-155，miR-196a，miR-203）的表达水平。使用定量实时PCR（qPCR）检测阳性宫颈增生和肿瘤组织样本。还分析了社会人口学和生活方式因素。结果：miR-27a在宫颈上皮内瘤变（CIN）2-3中的表达显着高于CIN1（p = 0.023），在鳞状细胞癌（SCC）中的表达显着高于CIN2-3（p = 0.033）。此外，在CIN2-3中检测到的miR-34a水平明显低于CIN1（p = 0.041）和SCC中的水平（与CIN2-3（p = 0.021）相比）。此外，我们发现在CIN2-3中miR-27a（p = 0.015）和miR-203（p = 0.025）中具有多个HPV的受试者与CIN1和miR-21（p = 0.002），mir-27a（ p = 0.001）和SCC / CIN2-3中的miR-34a（p = 0.001）。与CIN1（p = 0.028）和miR-34a（下调）相比，miR-27a的表达在CIN2-3中表达上调，与HPV 16阳性（CIN2-3 / CIN1：p = 0.027和SCC / CIN2-3：p ＝ 0.036）。与吸烟状态和HPV 16的存在有关，MiR-34a表达也发生了显着改变。结论：对具有预后潜力的其他替代分子生物标记的需求旺盛。评估miRNA的表达可能有助于区分不同的宫颈病变，并可能有助于预测HPV感染的结果。

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