Dendrofalconerol A Suppresses Migrating Cancer Cells via EMT and Integrin Proteins

Pengpaeng Premkamol; Sritularak Boonchoo; Chanvorachote Pithi

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Dendrofalconerol A Suppresses Migrating Cancer Cells via EMT and Integrin Proteins

机译：Dendrofalconerol A通过EMT和整合素蛋白抑制迁移的癌细胞

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Background/Aim: Enhanced cell motility is a hallmark of highly metastatic cancer cells. The anti-migratory activity of Dendrofalconerol A (DF-A), a pure bis(bibenzyls) isolated from the stem of Dendrobium falconeri (Orchidaceae) is reported in the present study. Materials and Methods: Cytotoxicity effects of DF-A on H460 lung cancer cells was determined by the MIT assay. We also investigated the mechanism of DF-A-mediated EMT and integrin proteins level by western blotting. Results: DF-A at concentrations of 0.5-5 mu M significantly reduced the protein level of migrating cells in a dose-dependent manner. The expression of migration-related integrins, including integrin beta(1) and integrin alpha(4) was significantly reduced in response to DF-A treatment. Also, DF-A was shown to suppress epithelial to mesenchymal transition (EMT), as indicated by cadherin switch from N- to E-cadherin and decrease of Snail, Slug and vimentin. Conclusion: This study revealed the potential of DF-A, an anti-metastasis agent and the underlying mechanism in this in vitro assay with H460 cells, which leads to the development of a novel anti-metastatic agent.

机译：背景/目的：增强细胞运动性是高度转移性癌细胞的标志。本研究报道了从铁皮石end（兰科）的茎中分离出的纯双（联苄基）Dendrofalconerol A（DF-A）的抗迁移活性。材料与方法：用MIT法测定DF-A对H460肺癌细胞的细胞毒性作用。我们还通过蛋白质印迹研究了DF-A介导的EMT和整合素蛋白水平的机制。结果：浓度为0.5-5μM的DF-A以剂量依赖性方式显着降低迁移细胞的蛋白质水平。迁移相关整联蛋白的表达，包括整联蛋白β（1）和整联蛋白α（4）大大减少响应DF-A处理。同样，如从钙粘蛋白从N-钙粘蛋白转换为E-钙粘蛋白并减少Snail，Slug和波形蛋白所示，DF-A还显示抑制上皮向间质转化（EMT）。结论：这项研究揭示了抗转移剂DF-A的潜力及其在H460细胞体外测定中的潜在机制，从而导致了新型抗转移剂的开发。

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《Anticancer Research: International Journal of Cancer Research and Treatment》 |2015年第1期|共5页
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Pengpaeng Premkamol; Sritularak Boonchoo; Chanvorachote Pithi;
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Dendrobium falconeri; dendrofalconerol A; EMT; integrin; suppression; migration; lung cancer;

机译：石end（Dendrobium falconeri）;dendrofalconerol A;EMT;integrin;抑制;迁移;肺癌;

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1. Dendrofalconerol A Suppresses Migrating Cancer Cells via EMT and Integrin Proteins [J] . Pengpaeng Premkamol, Sritularak Boonchoo, Chanvorachote Pithi Anticancer Research: International Journal of Cancer Research and Treatment . 2015,第1期

机译：Dendrofalconerol A通过EMT和整合素蛋白抑制迁移的癌细胞
2. Inhibition of TGF-β receptor I by siRNA suppresses the motility and invasiveness of T24 bladder cancer cells via modulation of integrins and matrix metalloproteinase [J] . Yubing Li, Kai Yang, Qiqi Mao, International Urology and Nephrology . 2010,第2期

机译：siRNA抑制TGF-β受体I通过调节整合素和基质金属蛋白酶抑制T24膀胱癌细胞的运动性和侵袭性
3. Long Intergenic Non-Protein Coding RNA 1089 Suppresses Cell Proliferation and Metastasis in Gastric Cancer by Regulating miRNA-27a-3p/Epithelial–Mesenchymal Transition (EMT) Axis [J] . Feng Yang, Xiaoting Chen, Xiyao Li, Cancer Management and Research . 2020,第4期

机译：通过调节miRNA-27A-3P /上皮 - 间充质转换（EMT）轴来抑制胃癌中细胞增殖和转移的长性非蛋白质编码RNA 1089
4. bcl-2 anti-apoptotic oncoprotein suppresses angiogenesis in non-small cell lung cancer: implications in resistance to photodynamic treatment? [C] . Author(s): M.I. Koukourakis Univ. Hospital of Iraklion (Greece) and Saint Savvas Hospital Iraklion Greece, A.Giatromanolaki Univ. Hospital of Iraklion (Greece) and Saint Savvas Hospital Iraklion Greece, J.Skarlatos Univ. Hospital of Iraklion (Greece) and Saint Savvas Hospital Iraklion Greece, GR-I international conference on new Laser technologies and applications . 1997

机译：bcl-2抗凋亡癌蛋白抑制非小细胞肺癌的血管生成：对光动力治疗的抵抗性有何影响？
5. Using integrins to fine tune CD4+ T cell responses: How the integrin regulatory proteins calpain, PIPKIgamma'90, talin and focal adhesion kinase work together to regulate CD4+ T cell activation. [D] . Wernimont, Sarah A. 2011

机译：使用整合素来微调CD4 + T细胞反应：整合素调节蛋白calpain，PIPKIgamma'90，talin和黏着斑激酶如何协同作用来调节CD4 + T细胞活化。
6. Inhibition of integrin-linked kinase (ILK) suppresses activation of protein kinase B/Akt and induces cell cycle arrest and apoptosis of PTEN-mutant prostate cancer cells [O] . Sujata Persad, Sarah Attwell, Virginia Gray, 2000

机译：整联蛋白连接激酶（ILK）的抑制抑制蛋白激酶B / Akt的激活并诱导PTEN突变的前列腺癌细胞的细胞周期停滞和凋亡
7. Inhibition of integrin-linked kinase (ILK) suppresses activation of protein kinase B/Akt and induces cell cycle arrest and apoptosis of PTEN-mutant prostate cancer cells [O] . Persad, Sujata, Attwell, Sarah, Gray, Virginia, 2000

机译：整联蛋白连接激酶（ILK）的抑制抑制蛋白激酶B / Akt的激活并诱导PTEN突变的前列腺癌细胞的细胞周期停滞和凋亡

Dendrofalconerol A Suppresses Migrating Cancer Cells via EMT and Integrin Proteins

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