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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Oral Administration of Lipopolysaccharide of Acetic Acid Bacteria Protects Pollen Allergy in a Murine Model
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Oral Administration of Lipopolysaccharide of Acetic Acid Bacteria Protects Pollen Allergy in a Murine Model

机译：口服乳酸菌脂多糖可保护小鼠模型中的花粉过敏

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Background/ Aim: Lipopolysaccharide (LPS), a major component of the cell wall of Gram-negative bacteria, is known to possess strong immune-regulatory activity. We have found and reported the existence of biologically-active LPS in acetic acid bacteria. The LPS shows Limulus-positive activity and activation of macrophages to produce nitric oxide and tumor necrosis factor. In this study, we investigated the anti-allergic effect of an orally-administrated acetic acid bacteria extract containing LPS; the cedar pollinosis model was used. Materials and Methods: Acetic acid bacteria were isolated from various fruits by Nodai kaihen medium. Then, the anti-allergic effect of acetic acid bacteria extracts was investigated. BALB/c mice were immunized with a mixture of cedar pollen and alum into their peritoneal cavity; they also received additional immunizations of pollen to nasal cavity. After immunizing the mice with pollen into their nasal cavity to trigger an allergic reaction, the frequency of nose scratching was counted for 5 min. Results: The bacteria were cultured and prepared and the water-extract contained about 1-10 mg/ml of Limulus positive substances. The extract of acetic acid bacteria induced higher levels of interleukin (IL)-10 and FOXP3 mRNA expression in macrophages (RAW246.7 cell), as assessed by DNA microarray analysis. Oral administration of the acetic acid bacteria extract demonstrated significantly less scratching numbers than control water group with pollen immunization. Conclusion: These results showed that LPS in acetic acid bacteria has the potential to protect from an allergic reaction, especially from cedar pollinosis.
机译：背景/目的：脂多糖（LPS）是革兰氏阴性细菌细胞壁的主要成分，已知具有强大的免疫调节活性。我们已经发现并报道了乙酸细菌中具有生物活性的LPS的存在。 LPS显示Li阳性和巨噬细胞活化，产生一氧化氮和肿瘤坏死因子。在这项研究中，我们研究了口服给予含有LPS的醋酸细菌提取物的抗过敏作用。使用雪松花粉病模型。材料与方法：通过Nodai kaihen培养基从各种水果中分离出乙酸细菌。然后，研究了乙酸细菌提取物的抗过敏作用。用雪松花粉和明矾的混合物将BALB / c小鼠免疫至腹膜腔。他们还接受了鼻腔花粉的额外免疫接种。用花粉免疫小鼠鼻腔以引发过敏反应后，计数鼻抓挠的频率5分钟。结果：培养并制备细菌，水提取物中含有约1-10 mg / ml的mul阳性物质。通过DNA芯片分析评估，乙酸细菌的提取物在巨噬细胞（RAW246.7细胞）中诱导了较高水平的白介素（IL）-10和FOXP3 mRNA表达。口服醋酸菌提取物显示，与花粉免疫的对照组相比，划伤次数明显少于对照组。结论：这些结果表明，乙酸细菌中的LPS具有防止过敏反应（特别是防止雪松花粉症）的潜力。

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