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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Pattern of IGF-1 Variants' Expression in Human Cancer Cell Lines Using a Novel q-RT-PCR Approach
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Pattern of IGF-1 Variants' Expression in Human Cancer Cell Lines Using a Novel q-RT-PCR Approach

机译：使用新型q-RT-PCR方法在人癌细胞系中IGF-1变体表达模式

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摘要

Background/Aim: Although insulin-like growth factor-1 (IGF-1) is well-implicated in cancer biology, the potential roles of IGF-1 variants in different types of cancer are largely unknown. The aim of the present study was to in vitro characterize several human cancers for their IGF-1 variant expression patterns. Materials and Methods: Using a novel quantitative real-time polymerase chain reaction (qRT-PCR) assay, twelve human cancer cell lines were investigated for their endogenous expression levels of IGF-1 variants, including classes. Additionally, the hormonal regulation of IGF-1 transcripts was investigated in PC3 cells. Results: IGF-1Ea and Eb were found at higher levels in KLE and MEL28 cells, respectively. MCF7 had the lowest expression of Ec peptide and, along with MB231, lacked IGF-1Eb. In most cases, class 1 proved as the predominant origin. Estradiol (E2) or dexamethasone (Dexa) significantly modulated IGF-1Ea and IGF-1Eb and down-regulated the Ec peptide in PC3. Conclusion: Our results contribute to the notion of distinct roles of IGF-1 isoforms in human cancer depending on the type of malignancy.
机译：背景/目的：尽管胰岛素样生长因子-1（IGF-1）在癌症生物学中有很好的关联，但IGF-1变体在不同类型癌症中的潜在作用尚不清楚。本研究的目的是针对几种人类癌症的IGF-1变异表达模式进行体外表征。材料和方法：使用新颖的实时定量聚合酶链反应（qRT-PCR）分析方法，研究了十二种人类癌细胞系的IGF-1变体（包括类别）的内源表达水平。此外，在PC3细胞中研究了IGF-1转录本的激素调节。结果：在KLE和MEL28细胞中，IGF-1Ea和Eb的含量较高。 MCF7具有最低的Ec肽表达，并且与MB231一起缺乏IGF-1Eb。在大多数情况下，1类被证明是主要血统。雌二醇（E2）或地塞米松（Dexa）显着调节了IGF-1Ea和IGF-1Eb，并下调了PC3中的Ec肽。结论：我们的结果有助于根据恶性肿瘤的类型，将IGF-1亚型在人类癌症中发挥独特作用。

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