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首页> 外文期刊>Antimicrobial agents and chemotherapy. >Reply to 'probability of eradication using vancomycin alone or in combination for methicillin-resistant Staphylococcus aureus bacteremia'
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Reply to 'probability of eradication using vancomycin alone or in combination for methicillin-resistant Staphylococcus aureus bacteremia'

机译：答复“单独使用万古霉素或联合使用可消除耐甲氧西林的金黄色葡萄球菌菌血症的可能性”

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摘要

Mass spectrometry is a widely used technique for protein identification and it has also become the method of choice in order to detect and characterize the post-translational modifications (PTMs) of proteins. Many software tools have been developed to deal with this complication. In this paper we introduce a new, free and user friendly online software tool, named POTAMOS Mass Spectrometry Calculator, which was developed in the open source application framework Ruby on Rails. It can provide calculated mass spectrometry data in a time saving manner, independently of instrumentation. In this web application we have focused on a well known protein family of histones whose PTMs are believed to play a crucial role in gene regulation, as suggested by the so called "histone code" hypothesis. The PTMs implemented in this software are: methylations of arginines and lysines, acetylations of lysines and phosphorylations of serines and threonines. The application is able to calculate the kind, the number and the combinations of the possible PTMs corresponding to a given peptide sequence and a given mass along with the full set of the unique primary structures produced by the possible distributions along the amino acid sequence. It can also calculate the masses and charges of a fragmented histone variant, which carries predefined modifications already implemented. Additional functionality is provided by the calculation of the masses of fragments produced upon protein cleavage by the proteolytic enzymes that are most widely used in proteomics studies.
机译：质谱分析是一种广泛用于蛋白质鉴定的技术，它也已成为检测和表征蛋白质翻译后修饰（PTM）的首选方法。已经开发了许多软件工具来应对这种复杂性。在本文中，我们介绍了一种名为POTAMOS质谱计算器的新的，免费且用户友好的在线软件工具，该工具是在开源应用程序框架Ruby on Rails中开发的。它可以以节省时间的方式提供计算出的质谱数据，而与仪器无关。在这个Web应用程序中，我们集中于一个众所周知的组蛋白蛋白质家族，其PTM被认为在基因调控中起着至关重要的作用，正如所谓的“组蛋白密码”假设所暗示的那样。在该软件中实现的PTM是：精氨酸和赖氨酸的甲基化，赖氨酸的乙酰化和丝氨酸和苏氨酸的磷酸化。该应用程序能够计算与给定的肽序列和给定的质量相对应的可能的PTM的种类，数量和组合，以及由沿着氨基酸序列的可能分布产生的整套独特的一级结构。它还可以计算片段化的组蛋白变体的质量和电荷，其中该组蛋白变体带有已实施的预定义修饰。通过计算蛋白质组学研究中最广泛使用的蛋白水解酶切割蛋白后产生的片段的质量，可以提供其他功能。

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