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首页> 外文期刊>Antimicrobial agents and chemotherapy. >Evaluation of clonality and carbapenem resistance mechanisms among Acinetobacter baumannii-Acinetobacter calcoaceticus complex and Enterobacteriaceae isolates collected in european and mediterranean countries and detection of two novel β-lactamases, GES-22 and VIM-35
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Evaluation of clonality and carbapenem resistance mechanisms among Acinetobacter baumannii-Acinetobacter calcoaceticus complex and Enterobacteriaceae isolates collected in european and mediterranean countries and detection of two novel β-lactamases, GES-22 and VIM-35

机译：评价欧洲和地中海国家收集的鲍曼不动杆菌-钙乙酸醋酸杆菌复合体和肠杆菌科细菌的克隆性和碳青霉烯耐药机制，并检测两种新型β-内酰胺酶GES-22和VIM-35

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摘要

We evaluated doripenem-resistant Acinetobacter baumannii-Acinetobacter calcoaceticus complex (ACB; n = 411) and Enterobacteriaceae (n=92) isolates collected from patients from 14 European and Mediterranean countries during 2009 to 2011 for the presence of carbapenemase-encoding genes and clonality. Following susceptibility testing, carbapenem-resistant (doripenem MIC, 2 μg/ml) isolates were screened for carbapenemases. New β-lactamase genes were expressed in a common background and susceptibility was tested. Class 1 integrons were sequenced. Clonality was evaluated by pulsed-field gel electrophoresis and multilocus sequence typing (Pasteur scheme). Relative expression of β-lactam intrinsic resistance mechanisms was determined for carbapenemase-negative Enterobacteriaceae. ACB and Enterobacteriaceae displayed 58.9 and 0.9% doripenem resistance, respectively. BlaOXA-23, blaOXA-58, and blaOXA-24/OXA-40 were detected among 277, 77, and 29 ACB, respectively (in 8, 6, and 5 countries). Ten Turkish isolates carried blaGES-11 or blaGES-22. GES-22 (G243A and M169L mutations in GES-1) had an extendedspectrum β-lactamase profile. A total of 33 clusters of 2 ACB isolates were observed, and 227 isolates belonged to sequence type 2/international clone II. Other international clones were limited to Turkey and Israel. Doripenem-resistant Enterobacteriaceae increased significantly (0.7 to 1.6%), and 15 blaKPC-2- and 22 blaKPC-3- carrying isolates, mostly belonging to clonal complexes 11 and 258, were observed. Enterobacteriaceae isolates producing OXA-48 (n=16; in Turkey and Italy), VIM-1 (n=10; in Greece, Poland, and Spain), VIM-26 (n=1; in Greece), and IMP-19, VIM-4, and the novel VIM-35 (n=1 each from Poland) were detected. VIM-35 had one substitution compared to VIM-1 (A235T) and a similar susceptibility profile. One or more resistance mechanisms were identified in 4/6 carbapenemase-negative Enterobacteriaceae. This broad evaluation confirms results from country-specific surveys and shows a highly diverse population of carbapenemase-producing ACB and Enterobacteriaceae in Europe and Mediterranean countries.
机译：我们评估了从2009年至2011年从14个欧洲和地中海国家的患者收集的耐多烯培南的鲍曼不动杆菌鲍曼不动杆菌钙乙酸复合物（ACB; n = 411）和肠杆菌科细菌（n = 92）分离物的碳青霉烯酶编码基因和克隆性。药敏试验后，筛选抗碳青霉烯类（多洛培南MIC，> 2μg/ ml）的碳青霉烯酶。在共同的背景下表达新的β-内酰胺酶基因，并测试了药敏性。对1类整合素测序。通过脉冲场凝胶电泳和多基因座序列分型（Pasteur方案）评估克隆性。确定了碳青霉烯酶阴性肠杆菌科细菌β-内酰胺固有抗性机制的相对表达。 ACB和肠杆菌科细菌分别显示出58.9％和0.9％的多烯培南耐药性。在277、77和29个ACB中分别检测到BlaOXA-23，blaOXA-58和blaOXA-24 / OXA-40（在8、6和5个国家/地区）。十个土耳其分离株携带blaGES-11或blaGES-22。 GES-22（GES-1中的G243A和M169L突变）具有广谱的β-内酰胺酶谱。总共观察到33个簇，> 2个ACB分离株，其中227个分离株属于序列2 /国际克隆II。其他国际克隆仅限于土耳其和以色列。耐多利培南的肠杆菌科细菌显着增加（0.7至1.6％），观察到15株携带blaKPC-2-和22 blaKPC-3-分离株，主要属于克隆复合体11和258。肠杆菌科分离株产生OXA-48（n = 16;在土耳其和意大利），VIM-1（n = 10;在希腊，波兰和西班牙），VIM-26（n = 1;在希腊）和IMP-19 ，VIM-4和新型VIM-35（n = 1，分别来自波兰）被检测到。与VIM-1（A235T）相比，VIM-35具有一个取代基，并且敏感性相似。在4/6碳青霉烯酶阴性肠杆菌科中鉴定出一种或多种耐药机制。这项广泛的评估证实了特定国家/地区调查的结果，并显示了欧洲和地中海国家/地区生产碳青霉烯酶的ACB和肠杆菌科的人群高度多样化。

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