• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied optics >Optical saturation measurements of fluorophores in solution with pulsed femtosecond excitation and two-dimensional CCD camera detection
【24h】

Optical saturation measurements of fluorophores in solution with pulsed femtosecond excitation and two-dimensional CCD camera detection

机译：飞秒脉冲激发和二维CCD相机检测溶液中荧光团的光学饱和度测量

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We present a new method for the measurement of saturation of the optical transition of fluorescent molecules in solution, which is based on detection with a CCD camera of a two-dimensional projection of the three-dimensional, spatially nonuniform fluorescence intensity distribution as generated in a bulk solution of the fluorophore by excitation with focused femtosecond optical pulses. Essential to the method is (a) a combination of information from a measurement in saturation and one not in saturation and (b) for the measurement in saturation, the simultaneous observation of both saturated and nonsaturated regions of the fluorescence intensity distribution. The experimental setup is straightforward and good agreement is found between the theory and the experimental data.#1997 Optical Society of America
机译：我们提出了一种测量溶液中荧光分子的光学跃迁饱和度的新方法，该方法基于使用CCD相机检测三维，空间不均匀荧光强度分布的二维投影的二维投影，该二维强度在通过聚焦的飞秒光脉冲激发而形成的荧光团整体溶液。该方法必不可少的是（a）结合来自饱和度测量和非饱和度测量的信息，以及（b）用于饱和度测量的信息，同时观察荧光强度分布的饱和和非饱和区域。实验装置简单明了，在理论和实验数据之间找到了很好的一致性。＃1997美国光学学会

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号