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首页> 外文期刊>Physica Scripta: An International Journal for Experimental and Theoretical Physics >Applications of the time-resolved two-colour two-photon excitation of UV fluorophores using femtosecond laser pulses
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Applications of the time-resolved two-colour two-photon excitation of UV fluorophores using femtosecond laser pulses

机译：飞秒激光脉冲时间分辨双色双光子激发紫外荧光团的应用

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摘要

A short overview of the principles and applications of the two-colour two-photon (2C2P)excitation of fluorescence by using femtosecond pulses is given. Fluorescence is generated bythe simultaneous absorption of an 800 nm photon and a 400 nm photon of overlapping laserbeams of a titanium:sapphire laser. Two examples of its application are presented: firstly, it isused to monitor the enzymatic cleavage of bovine serum albumin (BSA) by elastase. Thefluorescent amino acid tryptophan present in BSA is excited corresponding to an effectiveone-photon wavelength of 266 nm. Secondly, it is shown how one can utilize the differentpolarizations of the excited beams for determining the symmetry of the excited states ofmolecules, exemplarily shown for p-terphenyl in cyclohexane. Further applications andexperiments for 2C2P are suggested for using it in UV-fluorescence microscopy and fordetermining the properties of the electronic states of biomolecules by using differentlypolarized photons.
机译：简要概述了使用飞秒脉冲对荧光进行二色双光子（2C2P）激发的原理和应用。钛蓝宝石激光器的重叠激光束同时吸收800 nm光子和400 nm光子，从而产生荧光。给出了其应用的两个例子：首先，它被用于监测弹性蛋白酶对牛血清白蛋白（BSA）的酶促裂解。 BSA中存在的荧光氨基酸色氨酸被激发，对应于266 nm的有效单光子波长。其次，示出了如何利用激发束的不同偏振来确定分子的激发态的对称性，示例性地示出了对环己烷中的对-三联苯。建议将2C2P用于紫外荧光显微镜，并通过使用不同偏振的光子确定生物分子电子态的性质，进行进一步的应用和实验。

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