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Quantification of fluorophore concentration in tissue-simulating media by fluorescence measurements with a single optical fiber

机译：通过单根光纤的荧光测量定量组织模拟介质中的荧光团浓度

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摘要

Quantifying fluorescent compounds in turbid media such as tissue is made difficult by the effects of multiple scattering and absorption of the excitation and emission light. The approach that we used was to measure fluorescence using a single 200-mum optical fiber as both the illumination source and the detector. Fluorescence of aluminum phthalocyanine tetrasulfonate (AlPcS4) was measured over a wide range of fluorophore concentrations and optical properties in tissue-simulating phantoms. A root-mean-square accuracy of 10.6% in AlPcS4 concentration was attainable when fluorescence was measured either interstitially or at the phantom surface. The individual effects of scattering, absorption, and the scattering phase function on the fluorescence signal were also studied by experiments and Monte Carlo simulations. (C) 2003 Optical Society of America. [References: 24]
机译：由于激发光和发射光的多重散射和吸收，难以量化混浊介质（例如组织）中的荧光化合物。我们使用的方法是使用一根200微米的光纤作为照明源和检测器来测量荧光。铝酞菁四磺酸铝（AlPcS4）的荧光在组织模拟体模中的各种荧光团浓度和光学性质上进行了测量。当在间隙中或在幻影表面测量荧光时，AlPcS4浓度的均方根精度达到10.6％。还通过实验和蒙特卡洛模拟研究了散射，吸收和散射相位函数对荧光信号的单独影响。 （C）2003年美国眼镜学会。 [参考：24]

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