• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied optics >Scanning total internal reflection fluorescence microscopy under one-photon and two-photon excitation: image formation
【24h】

Scanning total internal reflection fluorescence microscopy under one-photon and two-photon excitation: image formation

机译：一光子和两光子激发下的扫描全内反射荧光显微镜：成像

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We propose a new type of total internal reflection fluorescence microscopy (TIRFM) called scanning TIRFM (STIRFM) that uses a focused ring-beam illumination and a high-numerical-aperture objective (NA=1.65). The evanescent field produced by the STIRFM is focused laterally, producing a small excitation volume that can induce a nonlinear effect such as two-photon absorption. Experimental images of CdSe quantum dot nanocrystals and Rhodamine 6G-doped microbeads show that good lateral and axial resolutions are achieved with the current setup. The theoretical simulation of the focal spot produced in STIRFM geometry shows that the focused evanescent field is split into two peaks because of the depolarization effect of a high numerical-aperture objective lens. However, the point-spread function analysis of both one-photon and two-photon excitation cases shows that the detection of the focus-splitting effect is dependent on the detection pinhole size. The effect of pinhole size on image formation is theoretically investigated and confirmed experimentally with the nanocrystal images.
机译：我们提出了一种新型的全内反射荧光显微镜（TIRFM），称为扫描TIRFM（STIRFM），它使用聚焦的环形光束照明和高数值孔径的物镜（NA = 1.65）。 STIRFM产生的渐逝场横向聚焦，产生小的激发体积，可以引起非线性效应，例如双光子吸收。 CdSe量子点纳米晶体和若丹明6G掺杂微珠的实验图像表明，使用当前设置可获得良好的横向和轴向分辨率。在STIRFM几何形状中产生的焦点的理论模拟表明，由于高数值孔径物镜的去极化作用，聚焦的e逝场被分为两个峰。但是，对单光子和两光子激发情况的点扩展函数分析表明，焦点分离效果的检测取决于检测针孔的大小。理论上研究了针孔尺寸对图像形成的影响，并通过纳米晶体图像进行了实验证实。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号