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Single-particle evanescent light scattering simulations for total internal reflection microscopy

机译:用于全内反射显微镜的单粒子e逝光散射模拟

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摘要

We simulate and measure light scattering of a micrometer-sized spherical particle suspended in solution close to a glass substrate. The model, based on the discrete sources method, is developed to describe the experimental situation of total internal reflection microscopy experiments; i.e., the particle is illuminated by an evanescent light field originating from the glass-solvent interface. In contrast to the well-established assumption of a simple exponential decay of the scattering intensity with distance, we demonstrate significant deviations for a certain range of penetration depths and polarization states of the incident light.
机译:我们模拟和测量悬浮在靠近玻璃基板的溶液中的微米级球形颗粒的光散射。建立了基于离散源方法的模型,以描述全内反射显微镜实验的实验情况;即,该颗粒被源自玻璃-溶剂界面的e逝光场照亮。与公认的散射强度随距离的简单指数衰减的公认假设相反,我们证明了一定深度的入射光的穿透深度和偏振态存在明显偏差。

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