• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied optics >Scanning flow cytometer modified to distinguish phytoplankton cells from their effective size, effective refractive index, depolarization, and fluorescence
【24h】

Scanning flow cytometer modified to distinguish phytoplankton cells from their effective size, effective refractive index, depolarization, and fluorescence

机译：修改扫描流式细胞仪以区分浮游植物细胞的有效大小，有效折射率，去极化和荧光

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A laser flow cytometer based on scanning flow cytometry has been assembled. The unpolarized and linearly polarized light-scattering profiles, as well as the side emitted light in different spectral bands, were measured, allowing the simultaneous and real-time determination of the effective size and the effective refractive index of each spherelike particle. Additionally, each particle could be identified from depolarization and fluorescence measured simultaneously. The tests with aqueous samples of polystyrene spheres, fluorescent or nonfluorescent, and phytoplankton cells demonstrate that the system is able to retrieve size and refractive index with an accuracy of 1percent and that the depolarization and fluorescence measurements allow the classification of particles otherwise indistinguishable.
机译：已经组装了基于扫描流式细胞仪的激光流式细胞仪。测量了非偏振和线性偏振的光散射曲线，以及在不同光谱带中的侧面发射光，从而可以同时实时地确定每个球形颗粒的有效尺寸和有效折射率。另外，可以从去极化和同时测量荧光来识别每个粒子。用聚苯乙烯球，荧光或无荧光以及浮游植物细胞的含水样品进行的测试表明，该系统能够以1％的准确度检索尺寸和折射率，并且去极化和荧光测量值可以使颗粒分类，否则无法区分。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号