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Divided-aperture technique for fluorescence confocal microscopy through scattering media

机译:通过散射介质的荧光共聚焦显微镜的分孔径技术

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摘要

We present a diffraction analysis for image formation in fluorescence confocal microscopy with divided apertures. The three-dimensional optical transform function is given, and axial resolution and transverse resolution are investigated. The results show that the employment of a divided-aperture technique in fluorescence confocal microscopy can enhance the rejection of background scattering. In addition, the axial resolution and transverse resolution can be improved by adjusting the width of the divider strip. For a given detector size, an optimum value of the divider strip width is given to obtain the best axial resolution or transverse resolution. The integrated intensity and signal-to-background ratio are also discussed.
机译:我们提出了在荧光共聚焦显微镜成像技术中形成孔径的衍射分析。给出了三维光学变换函数,并研究了轴向分辨率和横向分辨率。结果表明,在荧光共聚焦显微镜中采用分孔径技术可以增强对背景散射的抑制。另外,通过调节分隔带的宽度可以改善轴向分辨率和横向分辨率。对于给定的探测器尺寸,给出分隔带宽度的最佳值以获得最佳的轴向分辨率或横向分辨率。还讨论了积分强度和信噪比。

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