首页> 外文期刊>Applied optics >Development of a quantitative optical biochip based on a double integrating sphere system that determines absolute photon number in bioluminescent solution: application to quantum yield scale realization
【24h】

Development of a quantitative optical biochip based on a double integrating sphere system that determines absolute photon number in bioluminescent solution: application to quantum yield scale realization

机译:基于确定生物发光溶液中绝对光子数的双积分球系统的定量光学生物芯片的开发:​​在量子产率规模实现中的应用

获取原文
获取原文并翻译 | 示例
           

摘要

We report a new design of an optical biochip based on a double integrating sphere system to quantify the absolute number of the emitted photons or the total photon flux by a whole cell bioluminescent biosensor, for water toxicity detection, based on genetically engineered Escherichia coli bacteria carrying a recA::luxCDABE promoter-reporter fusion. The new design of the double integrating sphere system does not require any external standard light source for calibration of the tested bioluminescent solution and allows a direct determination of the total photon flux of the bioluminescent solution. In our design, we required that the two spheres are symmetric (have the same radius and reflectance) with a surface area larger than the cut cap area between the spheres.
机译:我们报告了一种基于双积分球系统的光学生物芯片的新设计,该技术可以通过基于基因工程的大肠杆菌细菌携带的全细胞生物发光生物传感器来量化所发射光子的绝对数量或总光子通量,从而用于水毒性检测recA :: luxCDABE启动子-报告子融合。双积分球系统的新设计不需要任何外部标准光源即可校准被测生物发光溶液,并可以直接确定生物发光溶液的总光子通量。在我们的设计中,我们要求两个球体对称(具有相同的半径和反射率),并且其表面积大于两个球体之间的切割帽面积。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号