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首页> 外文期刊>Applied optics >Depth-resolved velocimetry of Hagen-Poiseuille and electro-osmotic flow using dynamic phase-contrast microscopy
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Depth-resolved velocimetry of Hagen-Poiseuille and electro-osmotic flow using dynamic phase-contrast microscopy

机译：动态相衬显微镜对哈根-泊瓦的深度分辨测速和电渗流

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摘要

We quantitatively investigate the axial imaging properties of dynamic phase-contrast microscopy, with a special focus on typical combinations of tracer particles and magnifications that are used for velocimetry analysis. We show, for the first time, that a dynamic phase-contrast microscope, which is the integration of an all-optical novelty filter in a commercially available inverted microscope, can visualize three-dimensional velocity fields with a significantly reduced optical sectioning depth. The depth of field for dynamic phase-contrast microscopy is extracted from the three-dimensional response function and compared with the respective values for incoherent bright-field illumination. These results are then used to perform a depth-resolved particle image velocimetry analysis of Hagen-Poiseuille as well as electro-osmotically actuated flows in a microchannel.
机译：我们定量研究动态相差显微镜的轴向成像特性，特别关注示踪剂颗粒和测速分析中使用的放大倍率的典型组合。我们首次展示了动态相衬显微镜，它是在市场上可买到的倒置显微镜中集成了全光学新颖滤光片，可以可视化具有明显减小的光学切片深度的三维速度场。从三维响应函数中提取动态相衬显微镜的景深，并与非相干明场照明的相应值进行比较。然后将这些结果用于对Hagen-Poiseuille以及微通道中的电渗动流进行深度解析粒子图像测速分析。

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