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Measurement of two-photon excitation spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy

机译:非线性傅里叶变换光谱法测量荧光蛋白的双光子激发光谱

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摘要

We present measurements of two-photon excitation (TPE) spectra of various fluorescent proteins with nonlinear Fourier-transform spectroscopy. By using an ultrabroadband laser pulse with a spectrum ranging from 700 to 1100 nm, the absolute TPE spectra of six typical fluorescent proteins (SeBFP, Sapphire, eGFP, eCFP, Venus, DsRed) were measured with high spectral resolution.
机译:我们目前用非线性傅里叶变换光谱法测量各种荧光蛋白的双光子激发(TPE)光谱。通过使用光谱范围为700至1100 nm的超宽带激光脉冲,以高光谱分辨率测量了六个典型荧光蛋白(SeBFP,蓝宝石,eGFP,eCFP,Venus,DsRed)的绝对TPE光谱。

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