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Fluorescence volume imaging with an axicon: simulation study based on scalar diffraction method

机译:轴锥荧光体成像:基于标量衍射法的模拟研究

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摘要

In a two-photon excitation fluorescence volume imaging (TPFVI) system, an axicon is used to generate a Bessel beam and at the same time to collect the generated fluorescence to achieve large depth of field. A slice-by-slice diffraction propagation model in the frame of the angular spectrum method is proposed to simulate the whole imaging process of TPFVI. The simulation reveals that the Bessel beam can penetrate deep in scattering media due to its self-reconstruction ability. The simulation also demonstrates that TPFVI can image a volume of interest in a single raster scan. Two-photon excitation is crucial to eliminate the signals that are generated by the side lobes of Bessel beams; the unwanted signals may be further suppressed by placing a spatial filter in the front of the detector. The simulation method will guide the system design in improving the performance of a TPFVI system.
机译:在双光子激发荧光体积成像(TPFVI)系统中,轴锥仪用于产生贝塞尔光束,同时收集产生的荧光以实现大景深。提出了一种在角谱方法框架内逐层衍射传播模型,以模拟TPFVI的整个成像过程。仿真表明,贝塞尔光束具有自我重建的能力,可以穿透散射介质深处。仿真还表明,TPFVI可以在一次光栅扫描中对感兴趣的体积成像。双光子激发对于消除由贝塞尔光束旁瓣产生的信号至关重要。通过在检测器的前面放置一个空间滤波器,可以进一步抑制有害信号。仿真方法将指导系统设计提高TPFVI系统的性能。

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