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首页> 外文期刊>Applied optics >Measurement of membrane rigidity on trapped unilamellar phospholipid vesicles by using differential confocal microscopy
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Measurement of membrane rigidity on trapped unilamellar phospholipid vesicles by using differential confocal microscopy

机译：使用差动共聚焦显微镜测量捕获的单层磷脂囊泡的膜刚度

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摘要

We use optical tweezers to trap a unilamellar phospholipid vesicle and measure the out-of-plane thermal fluctuations by using differential confocal microscopy. Bending moduli of the lipid membranes are calculated directly from the mean-square values of the fluctuation amplitudes. Owing to the refractive index contrast between the inner and outer solutions of the vesicle, optical tweezers trap the vesicle laterally and improve the reliability of the measured fluctuation amplitudes along the optical axis. Bending moduli of membranes in gel or fluid phases obtained by the combination of differential confocal microscopy and optical tweezers are close to those reported previously. We also obtain the bending modulus of sphingomyelin membranes in the gel phase, which was not reported previously.
机译：我们使用光学镊子捕获单层磷脂囊泡，并通过使用差分共聚焦显微镜测量平面外的热波动。脂质膜的弯曲模量直接根据波动幅度的均方值来计算。由于囊的内部溶液和外部溶液之间的折射率对比，光镊将囊横向捕获，并提高了沿光轴测量的波动幅度的可靠性。通过差动共聚焦显微镜和光学镊子的组合获得的凝胶或液相中膜的弯曲模量与先前报道的相近。我们还获得了鞘磷脂膜在凝胶相中的弯曲模量，这是以前没有报道的。

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