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Modeling of fiber-optic fluorescence probes for strongly absorbing samples

机译：用于强吸收样品的光纤荧光探针的建模

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摘要

The dynamic range of fiber-optic fluorescent probes such as single fibers and fiber bundles is calculated for strongly absorbing samples, such as process liquids, foodstuffs, and lubricants. The model assumes an excitation beam profile based on a Lambertian light source and uses analytical forms of the collection efficiency, followed by an Abel transformation and numerical integration. It is found that the effect of primary absorption of the excitation light and secondary absorption of the fluorescence is profound. For fiber bundles and bifurcated fiber probes, the upper accessible concentration limit is roughly given by the absorption length of the primary and secondary absorption. Fluorescence detectors that are placed at right angles to the excitation beam axis or collinear to the beam axis are equally strongly affected by secondary absorption. A probe in which the same fiber is used for excitation and for collection of the fluorescence emerges as the fiber probe with the largest accessible concentration range.
机译：计算用于强吸收样品（例如处理液，食品和润滑剂）的光纤荧光探针（例如单纤维和纤维束）的动态范围。该模型假设基于朗伯光源的激发光束轮廓，并使用收集效率的分析形式，然后进行Abel变换和数值积分。发现激发光的一次吸收和荧光的二次吸收的影响是深远的。对于光纤束和分叉的光纤探头，可达到的浓度上限大致由一次和二次吸收的吸收长度给出。与激发光束轴成直角或与光束轴共线放置的荧光检测器同样受到二次吸收的强烈影响。作为具有最大可及浓度范围的光纤探针，出现了使用相同光纤进行激发和收集荧光的探针。

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