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Parallel on-axis phase-shifting holographic phase microscopy based on reflective point-diffraction interferometer with long-term stability

机译:基于反射点衍射干涉仪的具有长期稳定性的平行轴相移全息相显微镜

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摘要

Parallel on-axis two-step phase-shifting reflective point-diffraction interferometry for holographic phase microscopy based on Michelson architecture is proposed. A cube beamsplitter splits the object wave into two copies within the two arms. The reference wave is rebuilt by low-pass filtering with a pinhole-masked mirror. Both object and reference waves are split into two beams by a grating in a 4f imaging system; thus, two interferograms with quadrature phase-shift can be acquired simultaneously with the aid of polarization elements. The approach has the merit of nanometers-scale phase stability over hours due to its quasi-common-path geometry. It can make full use of camera spatial bandwidth while its temporal resolution is as fast as the camera frame rate. Phase imaging on microscale specimen is implemented, and the experimental results demonstrate that the proposed approach is suitable for investigating dynamic processes.
机译:提出了一种基于迈克尔逊体系结构的全息相显微镜平行轴两步相移反射点衍射干涉仪。立方体分光镜将物波在两个臂内分成两个副本。参考波通过带针孔掩盖镜的低通滤波重建。在4f成像系统中,物体波和参考波都被光栅分成两束;因此,借助偏振元件可以同时获取两个具有正交相移的干涉图。该方法由于具有准共路径几何形状,因此具有在数小时内具有纳米级相位稳定性的优点。它可以充分利用相机的空间带宽,同时其时间分辨率与相机的帧速率一样快。实现了对微尺度标本的相位成像,实验结果表明,该方法适用于研究动态过程。

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