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Field-based dynamic light scattering microscopy: theory and numerical analysis

机译：基于场的动态光散射显微镜：理论和数值分析

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摘要

We present a theoretical framework for field-based dynamic light scattering microscopy based on a spectral-domain optical coherence phase microscopy (SD-OCPM) platform. SD-OCPM is an interferometric microscope capable of quantitative measurement of amplitude and phase of scattered light with high phase stability. Field-based dynamic light scattering (F-DLS) analysis allows for direct evaluation of complex-valued field autocorrelation function and measurement of localized diffusive and directional dynamic properties of biological and material samples with high spatial resolution. In order to gain insight into the information provided by F-DLS microscopy, theoretical and numerical analyses are performed to evaluate the effect of numerical aperture of the imaging optics. We demonstrate that sharp focusing of fields affects the measured diffusive and transport velocity, which leads to smaller values for the dynamic properties in the sample. An approach for accurately determining the dynamic properties of the samples is discussed.
机译：我们提出了基于光谱域光学相干相显微镜（SD-OCPM）平台的基于场的动态光散射显微镜的理论框架。 SD-OCPM是一种干涉显微镜，能够以高相位稳定性定量测量散射光的振幅和相位。基于场的动态光散射（F-DLS）分析允许直接评估复值场自相关函数，并以高空间分辨率测量生物和材料样品的局部扩散和方向动态特性。为了深入了解F-DLS显微镜提供的信息，进行了理论和数值分析以评估成像光学元件的数值孔径的影响。我们证明了场的聚焦会影响所测量的扩散速度和传输速度，从而导致样品中动态特性的值较小。讨论了一种准确确定样品动力学特性的方法。

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