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Fabry-Perot-based Fourier-transform hyperspectral imaging allows multi-labeled fluorescence analysis

机译:基于法布里-珀罗的傅立叶变换高光谱成像可进行多标记荧光分析

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摘要

We demonstrate the ability of our hyperspectral imaging device, based on a scanning Fabry-Perot interferometer, to obtain a single hyper-image of a sample marked with different fluorescent molecules, and to unambiguously discriminate them by observing their spectral fingerprints. An experiment carried out with cyanines, fluorescein, and quantum dots emitting in the yellow-orange region, demonstrates the feasibility of multi-labeled fluorescence microscopy without the use of multiple filter sets or dispersive means.
机译:我们基于扫描法布里-珀罗干涉仪展示了我们的高光谱成像设备能够获得标有不同荧光分子的样品的单张超图像,并通过观察其光谱指纹来明确区分它们的能力。用花青素,荧光素和在橙黄色区域发射的量子点进行的实验表明,无需使用多个滤光片组或分散装置即可进行多标记荧光显微镜检查。

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