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Three-dimensional quantitative phase imaging via tomographic deconvolution phase microscopy

机译：通过层析反褶积相显微镜进行三维定量相成像

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The field of three-dimensional quantitative phase imaging (3D QPI) is expanding rapidly with applications in biological, medical, and industrial research, development, diagnostics, and metrology. Much of this research has centered on developing optical diffraction tomography (ODT) for biomedical applications. In addition to technical difficulties associated with coherent noise, ODT is not congruous with optical microscopy utilizing partially coherent light, which is used in most biomedical laboratories. Thus, ODT solutions have, for the most part, been limited to customized optomechanical systems which would be relatively expensive to implement on a wide scale. In the present work, a new phase reconstruction method, called tomographic deconvolution phase microscopy (TDPM), is described which makes use of commercial microscopy hardware in realizing 3D QPI. TDPM is analogous to methods used in deconvolution microscopy which improve spatial resolution and 3D-localization accuracy of fluorescence micrographs by combining multiple through-focal scans which are deconvolved by the system point spread function. TDPM is based on the 3D weak object transfer function theory which is shown here to be capable of imaging "nonweak" phase objects with large phase excursions. TDPM requires no phase unwrapping and recovers the entire object spectrum via object rotation, mitigating the need to fill in the "missing cone" of spatial frequencies algorithmically as in limited-angle ODT. In the present work, TDPM is demonstrated using optical fibers, including single-mode, polarization-maintaining, and photonic-crystal fibers as well as an azimuthally varying CO2-laser-induced long-period fiber grating period as test phase objects. (C) 2015 Optical Society of America
机译：随着在生物学，医学和工业研究，开发，诊断和计量学中的应用，三维定量相位成像（3D QPI）领域正在迅速扩展。这项研究的大部分集中在为生物医学应用开发光学衍射断层扫描（ODT）。除了与相干噪声相关的技术难题外，ODT与使用部分相干光的光学显微镜也不相符，在大多数生物医学实验室中都使用了部分相干光。因此，ODT解决方案在大多数情况下仅限于定制的光机械系统，而大规模实施的成本相对较高。在当前的工作中，描述了一种称为断层扫描反卷积相显微镜（TDPM）的新相重建方法，该方法利用商用显微镜硬件实现3D QPI。 TDPM类似于反卷积显微镜中使用的方法，该方法通过结合多个通过系统点扩散函数进行反卷积的贯穿焦点扫描，来提高荧光显微照片的空间分辨率和3D定位精度。 TDPM基于3D弱对象传递函数理论，此处显示出能够对具有大相位偏移的“非弱”相位对象进行成像。 TDPM不需要相位解缠，并且可以通过物体旋转来恢复整个物体光谱，从而减少了像有限角度ODT那样通过算法填充空间频率的“缺失圆锥”的需求。在目前的工作中，使用包括单模光纤，保偏光纤和光子晶体光纤以及方位角变化的CO2激光诱导的长周期光纤光栅周期作为测试相对象，来演示TDPM。 （C）2015年美国眼镜学会

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