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首页> 外文期刊>Asian Journal of Chemistry: An International Quarterly Research Journal of Chemistry >Separation and Analysis of Seven Kind Compounds in Three Medicine Herbs Extraction by RP-HPLC
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Separation and Analysis of Seven Kind Compounds in Three Medicine Herbs Extraction by RP-HPLC

机译：RP-HPLC分离分析三种药材中的七种化合物

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摘要

Saururus chinensis, Polygalae radix and Curcuma longa are widely known as traditional oriental medicinal herbs with various pharmacological activities. These three herbs were efficiently identified with 60 % aqueous ethanol and then successively partitioned with hexane, dichloromethane, ethyl acetate, normal butyl alcohol and water to investigate the seven compounds rutin, quercitrin, quercetin, onjisaponin B, bisdemethoxycurcumin, demethoxycurcumin and curcumin. The samples of these medicine herbs were simultaneously separated and analyzed by commercial C_(18) reverse phase high-performance liquid chromatography using the combination of water/ trifluoroacetic acid and acetonitrile as the mobile phase in the isocratic and gradient elution mode. The analysis conditions were as follows: flow rate 1 mL/min, injection volume 10 μL and column oven temperature 40 °C at 200-400 nm wavelengths. The yield (%) and total extraction amount (mg) of n-BuOH (11.88-18.80 %, 1.2-9.4 mg) were higher than those of the other partition solvents hexane. dichloromethane and ethyl acetate. Also, the optimum mobile phase was simultaneously separated and analyzed. These results suggest that the extracts of these three medicinal herbs can be good therapeutic materials for anti-oxidant and anti-angiogenesis activities in the treatment of obesity.
机译：龙，远志和姜黄被广泛认为是具有各种药理活性的传统东方草药。用60％的乙醇水溶液有效地鉴定了这三种草药，然后依次用己烷，二氯甲烷，乙酸乙酯，正丁醇和水分配，以研究7种化合物芦丁，槲皮素，槲皮素，onjisaponin B，双脱甲氧基姜黄素，脱甲氧基姜黄素和姜黄素。这些药材的样品同时分离，并通过水/三氟乙酸和乙腈的组合作为流动相，在等度和梯度洗脱模式下通过商业C_（18）反相高效液相色谱法进行分析。分析条件如下：流速为1 mL / min，进样量为10μL，柱温箱温度为200-400 nm波长下的40°C。正丁醇（11.88-18.80％，1.2-9.4 mg）的收率（％）和总提取量（mg）高于其他分配溶剂己烷的正丁醇。二氯甲烷和乙酸乙酯。另外，同时分离和分析了最佳流动相。这些结果表明，这三种草药的提取物可以作为用于治疗肥胖症的抗氧化和抗血管生成活性的良好治疗材料。

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