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Concentration-dependent fluorescence live-cell imaging and tracking of intracellular nanoparticles

机译:浓度依赖性荧光活细胞成像和细胞内纳米颗粒跟踪

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摘要

Using live-cell imaging techniques we investigated concentration-dependent intracellular movements of fluorescence nanoparticles (NPs) in real-time after their entry into HeLa cells via incubation. Intracellular particle traces appeared to be a mixture of both random and fairly unidirectional movements of the particles. At rather low concentrations of NPs, a majority of the non-random intracellular particle trajectories are assumed to mostly go along microtubule networks after endocytosis, as evidenced from the inhibition test with nocodazole. On the other hand, as the concentrations of NPs increased, random motions were more frequently observed inside the cells.
机译:使用活细胞成像技术,我们研究了荧光纳米颗粒(NPs)通过孵育进入HeLa细胞后实时的浓度依赖性细胞内运动。细胞内颗粒痕迹似乎是颗粒的随机和相当单向运动的混合物。从诺考达唑的抑制试验可以看出,在相当低的NP浓度下,大多数非随机的细胞内颗粒轨迹被认为在胞吞后大部分沿着微管网络运动。另一方面,随着NP浓度的增加,在细胞内部观察到随机运动的频率更高。

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