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首页> 外文期刊>Nanotechnology >Human haptoglobin phenotypes and concentration determination by nanogold-enhanced electrochemical impedance spectroscopy
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Human haptoglobin phenotypes and concentration determination by nanogold-enhanced electrochemical impedance spectroscopy

机译：人体触珠蛋白表型和纳米金增强电化学阻抗谱法测定浓度

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摘要

Haptoglobin (Hp) is an acute phase protein that binds free hemoglobin (Hb), preventing Hb-induced oxidative damage in the vascular system. There are three phenotypes in human Hp, whose heterogeneous polymorphic structures and varying concentrations in plasma have been attributed to the cause of diseases and outcome of clinical treatments. Different phenotypes of Hp may be composed of the same subunits but different copy numbers, rendering their determination difficult by a single procedure. In this study, we have developed a simple, fast, reliable and sensitive method, using label-free nanogold-modified bioprobes coupled with self-development electrochemical impedance spectroscopy (EIS). By this method, probe surface charge transfer resistance is detected. The relative charge transfer resistance ratios for Hp 1-1, Hp 2-1 and Hp 2-2 were characterized. We were able to determine protein size difference within 3nm, and the linear region of the calibration curve for Hp levels in the range of 90pgml~(- 1) and 90νgml~(- 1) (～1fM to 1pM). We surmise that similar approaches can be used to investigate protein polymorphism and altered protein-protein interaction associated with diseases.
机译：肝红蛋白（Hp）是一种结合自由血红蛋白（Hb）的急性期蛋白，可防止Hb诱导的血管系统氧化损伤。人类Hp中存在三种表型，其表型异质多态性和血浆中浓度的变化归因于疾病的原因和临床治疗的结果。 Hp的不同表型可能由相同的亚基组成，但拷贝数不同，因此很难通过单一程序确定它们。在这项研究中，我们开发了一种简单，快速，可靠和灵敏的方法，它使用了无标记的纳米金修饰的生物探针，并结合了自行开发的电化学阻抗谱（EIS）。通过这种方法，检测了探针表面电荷转移电阻。表征了Hp 1-1，Hp 2-1和Hp 2-2的相对电荷转移电阻比。我们能够确定3nm内的蛋白质大小差异，并且Hp水平的校准曲线的线性区域在90pgml〜（-1）和90νgml〜（-1）（〜1fM到1pM）之间。我们推测类似的方法可以用来研究蛋白质多态性和与疾病相关的蛋白质-蛋白质相互作用的改变。

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