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Mechanical properties and in vitro behavior of nanofiberhydrogel composites for tissue engineering applications

机译：用于组织工程的纳米纤维水凝胶复合材料的力学性能和体外行为

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摘要

Hydrogel-based biomaterial systems have great potential for tissue reconstruction by serving as temporary scaffolds and cell delivery vehicles for tissue engineering (TE). Hydrogels have poor mechanical properties and their rapid degradation limits the development and application of hydrogels in TE. In this study, nanofiber reinforced composite hydrogels were fabricated by incorporating electrospun poly(-caprolactone) (PCL)/gelatin blend or coaxial nanofibers into gelatin hydrogels. The morphological, mechanical, swelling and biodegradation properties of the nanocomposite hydrogels were evaluated and the results indicated that the moduli and compressive strengths of the nanofiber reinforced hydrogels were remarkably higher than those of pure gelatin hydrogels. By increasing the amount of incorporated nanofibers into the hydrogel, the Youngs modulus of the composite hydrogels increased from 3.291.02kPa to 20.301.79kPa, while the strain at break decreased from 66.01.1% to 52.03.0%. Compared to composite hydrogels with coaxial nanofibers, those with blend nanofibers showed higher compressive strength and strain at break, but with lower modulus and energy dissipation properties. Biocompatibility evaluations of the nanofiber reinforced hydrogels were carried out using bone marrow mesenchymal stem cells (BM-MSCs) by cell proliferation assay and immunostaining analysis. The nanocomposite hydrogel with 25mgml ~1 PCL/gelatin blend nanofibers (PGB25) was found to enhance cell proliferation, indicating that the nanocomposite hydrogels might provide the necessary mechanical support and could be promising cell delivery systems for tissue regeneration.
机译：基于水凝胶的生物材料系统通过用作组织工程（TE）的临时支架和细胞递送载体，具有巨大的组织重建潜力。水凝胶的机械性能较差，其快速降解限制了水凝胶在TE中的开发和应用。在这项研究中，纳米纤维增强复合水凝胶是通过将电纺聚己内酯（PCL）/明胶共混物或同轴纳米纤维掺入明胶水凝胶中制成的。评价了纳米复合水凝胶的形态，机械，膨胀和生物降解性能，结果表明，纳米纤维增强水凝胶的模量和压缩强度明显高于纯明胶水凝胶。通过增加掺入水凝胶中的纳米纤维的量，复合水凝胶的杨氏模量从3.291.02kPa增加到20.301.79kPa，而断裂应变从66.01.1％降低到52.03.0％。与具有同轴纳米纤维的复合水凝胶相比，具有共混纳米纤维的复合水凝胶显示出较高的抗压强度和断裂应变，但具有较低的模量和能量耗散性能。纳米纤维增强水凝胶的生物相容性评估是通过骨髓间充质干细胞（BM-MSC）通过细胞增殖测定和免疫染色分析进行的。发现具有25mgml〜1 PCL /明胶共混纳米纤维（PGB25）的纳米复合水凝胶可增强细胞增殖，表明纳米复合水凝胶可能提供必要的机械支持，并有望成为组织再生的有希望的细胞递送系统。

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