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Surface-biofunctionalized multicore/shell CdTe@SiO_2 composite particles for immunofluorescence assay

机译：表面生物功能化的多核/壳CdTe @ SiO_2复合颗粒用于免疫荧光测定

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摘要

Strongly fluorescent multicore/shell structured CdTe@SiO_2 composite particles of ～ 50nm were synthesized via the reverse microemulsion method by using CdTe quantum dots co-stabilized by thioglycolic acid and thioglycerol. The optical stability of the CdTe@SiO_2 composite particles in a wide pH range, under prolonged UV irradiation in pure water, or in different types of physiological buffers was systematically investigated. Towards immunofluorescence assay, both poly(ethylene glycol) (PEG) and carboxyl residues were simultaneously grafted on the surface of the silanol-terminated CdTe@SiO_2 composite particles upon further reactions with silane reagents bearing a PEG segment and carboxyl group, respectively, in order to suppress the nonspecific interactions of the silica particles with proteins and meanwhile introduce reactive moieties to the fluorescent particles. Agarose gel electrophoresis, dynamic light scattering and conventional optical spectroscopy were combined to investigate the effectiveness of the surface modifications. Via the surface carboxyl residue, various antibodies were covalently conjugated to the fluorescent particles and the resultant fluorescent probes were used in detecting cancer cells through both direct fluorescent antibody and indirect fluorescent antibody assays, respectively.
机译：利用巯基乙酸和巯基甘油共同稳定的CdTe量子点，通过反向微乳液法合成了约50nm的强荧光多核/壳结构CdTe @ SiO_2复合颗粒。系统地研究了CdTe @ SiO_2复合粒子在宽pH范围，长时间在纯水中或不同类型的生理缓冲液中的紫外线下的光学稳定性。进行免疫荧光测定时，分别与分别带有PEG段和羧基的硅烷试剂进一步反应，聚乙二醇（PEG）和羧基残基同时被接枝在硅烷醇封端的CdTe @ SiO_2复合颗粒的表面上。以抑制二氧化硅颗粒与蛋白质的非特异性相互作用，同时将反应性部分引入荧光颗粒。琼脂糖凝胶电泳，动态光散射和常规光学光谱相结合，以研究表面改性的有效性。经由表面羧基残基，将各种抗体共价缀合至荧光颗粒，并且所得的荧光探针分别用于通过直接荧光抗体测定和间接荧光抗体测定来检测癌细胞。

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