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首页> 外文期刊>Nanotechnology >Cellular imaging using biocompatible dendrimer-functionalized graphene oxide-based fluorescent probe anchored with magnetic nanoparticles
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Cellular imaging using biocompatible dendrimer-functionalized graphene oxide-based fluorescent probe anchored with magnetic nanoparticles

机译：使用生物相容性树状大分子官能化的基于氧化石墨烯的荧光探针锚定磁性纳米粒子的细胞成像

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摘要

We describe a novel multicomponent graphene nanostructured system that is biocompatible, and has strong NIR optical absorbance and superparamagnetic properties. The fabrication of the multicomponent nanostructure system involves the covalent attachment of 3 components; Fe _3O _4(Fe) nanoparticles, PAMAM-G4-NH _2 (G4) dendrimer and Cy5 (Cy) on a graphene oxide (GO) surface to synthesize a biologically relevant multifunctional system. The resultant GO-G4-Fe-Cy nanosystem exhibits high dispersion in an aqueous medium, and is magnetically responsive and fluorescent. Invitro experiments provide a clear indication of successful uptake of the GO-G4-Fe-Cy nanosystem by MCF-7 breast cancer cells, and it is seen to behave as a bright and stable fluorescent marker. The study also reveals varied cellular distribution kinetics profile for the GO nanostructured system compared to free Cy. Furthermore, the newly developed GO nanostructured system is observed to be non-toxic to MDA-MB-231 cell growth, in striking contrast to free G4 dendrimer and GO-G4 conjugate. The GO-G4-Fe-Cy nanostructured system characterized by multifunctionality suggests the merits of graphene for cellular bioimaging and the delivery of bioactives.
机译：我们描述了一种新型的生物相容的多组分石墨烯纳米结构系统，并具有很强的近红外光吸收和超顺磁性质。多组分纳米结构系统的制造涉及3个组分的共价连接;即3个组分。 Fe _3O _4（Fe）纳米粒子，PAMAM-G4-NH _2（G4）树枝状聚合物和Cy5（Cy）在氧化石墨烯（GO）表面上合成具有生物学意义的多功能系统。所得的GO-G4-Fe-Cy纳米系统在水性介质中显示出高分散性，并且具有磁响应性和荧光性。体外实验提供了MCF-7乳腺癌细胞成功摄取GO-G4-Fe-Cy纳米系统的明确指示，并且可以看作是明亮而稳定的荧光标记。这项研究还揭示了与游离Cy相比，GO纳米结构系统的细胞分布动力学变化。此外，观察到新开发的GO纳米结构系统对MDA-MB-231细胞生长无毒，与游离G4树状聚合物和GO-G4共轭物形成鲜明对比。具有多功能性的GO-G4-Fe-Cy纳米结构系统表明石墨烯在细胞生物成像和生物活性物质传递方面的优势。

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