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首页> 外文期刊>Nanotechnology >The synthesis and characterization of poly(γ-glutamic acid)-coated magnetite nanoparticles and their effects on antibacterial activity and cytotoxicity
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The synthesis and characterization of poly(γ-glutamic acid)-coated magnetite nanoparticles and their effects on antibacterial activity and cytotoxicity

机译：聚（γ-谷氨酸）包覆磁铁矿纳米粒子的合成，表征及其对抗菌活性和细胞毒性的影响

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摘要

Magnetite nanoparticles (MNPs) modified with sodium and calcium salts of poly(γ-glutamic acid) (NaPGA and CaPGA) were synthesized by the coprecipitation method, followed by characterization and evaluation of their antibacterial and cytotoxic effects. Superparamagnetic MNPs are particularly attractive for magnetic driving as well as bacterial biofilm and cell targeting in in vivo applications. Characterization of synthesized MNPs by the Fourier transform infrared spectra and magnetization curves confirmed the PGA coating on MNPs. The mean diameter of NaPGA-and CaPGA-coated MNPs as determined by transmission electron microscopy was 11.8 and 14 nm, respectively, while the x-ray diffraction pattern revealed the as-synthesized MNPs to be pure magnetite. Based on agar dilution assay, both NaPGA-and CaPGA-coated MNPs showed a lower minimum inhibitory concentration in Salmonella enteritidis SE 01 than the commercial antibiotics linezolid and cefaclor, but the former was effective against Escherichia coli ATCC 8739 and Staphylococcus aureus ATCC 10832, whereas the latter was effective against Escherichia coli O157:H7 TWC 01. An in vitro cytotoxicity study in human skin fibroblast cells as measured by MTT assay implied the as-synthesized MNPs to be nontoxic. This outcome demonstrated that both γ-PGA-modified MNPs are cytocompatible and possess antibacterial activity in vitro, and thereby should be useful in in vivo studies for biomedical applications.
机译：通过共沉淀法合成了用聚（γ-谷氨酸）的钠盐和钙盐（NaPGA和CaPGA）改性的磁铁矿纳米颗粒（MNP），然后表征和评估其抗菌和细胞毒性作用。在体内应用中，超顺磁性MNP对于磁性驱动以及细菌生物膜和细胞靶向特别有吸引力。通过傅立叶变换红外光谱和磁化曲线表征合成的MNP，证实了MNP上的PGA涂层。通过透射电子显微镜测定的NaPGA和CaPGA涂层的MNP的平均直径分别为11.8和14nm，而X射线衍射图显示合成后的MNP为纯磁铁矿。根据琼脂稀释试验，与商业抗生素利奈唑胺和头孢克洛相比，NaPGA和CaPGA包覆的MNPs在肠炎沙门氏菌SE 01中的最低抑菌浓度均较低，但前者对大肠杆菌ATCC 8739和金黄色葡萄球菌ATCC 10832有效，而后者对大肠杆菌O157：H7 TWC 01有效。通过MTT分析对人皮肤成纤维细胞进行的体外细胞毒性研究表明，合成后的MNP无毒。该结果表明，两种γ-PGA修饰的MNP均具有细胞相容性，并在体外具有抗菌活性，因此应在生物医学应用的体内研究中有用。

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