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Synthesis and characterization of model silica-gold core-shell nanohybrid systems to demonstrate plasmonic enhancement of fluorescence

机译:二氧化硅-金核壳纳米混合模型体系的合成与表征,证明荧光的等离子体增强

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In this work, gold-silica plasmonic nanohybrids have been synthesized as model systems which enable tuning of dye fluorescence enhancement/quenching interactions. For each system, a dye-doped silica core is surrounded by a 15nm spacer region, which in turn is surrounded by gold nanoparticles (GNPs). The GNPs are either covalently conjugated via mercapto silanization to the spacer or encapsulated in a separate external silica shell. The intermediate spacer region can be either dye doped or left undoped to enable quenching and plasmonic enhancement effects respectively. The study indicates that there is a larger enhancement effect when GNPs are encapsulated in the outer shell compared to the system of external conjugation. This is due to the environmental shielding provided by shell encapsulation compared to the exposure of the GNPs to the solvent environment for the externally conjugated system. The fluorescence signal enhancement of the nanohybrid systems was evaluated using a standard HRP-anti-HRP fluorescence based assay platform.
机译:在这项工作中,已合成了金-二氧化硅等离激元纳米杂交体作为模型系统,可以调节染料荧光增强/猝灭相互作用。对于每个系统,染料掺杂的二氧化硅核被15nm的间隔区包围,而间隔区又被金纳米颗粒(GNP)围绕。 GNP可以通过巯基硅烷化共价结合到间隔基上,也可以封装在单独的外部二氧化硅壳中。中间间隔区可以掺杂染料或不掺杂以分别实现淬灭和等离子体增强作用。研究表明,与外部共轭体系相比,将GNPs封装在外壳中具有更大的增强作用。这是由于与GNP暴露于外部共轭体系的溶剂环境相比,外壳封装提供了环境屏蔽。使用基于标准HRP-抗HRP荧光的分析平台评估了纳米杂化系统的荧光信号增强。

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