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Dielectrophoretic concentration of low-abundance nanoparticles using a nanostructured tip

机译：使用纳米结构尖端对低丰度纳米颗粒进行介电泳浓缩

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摘要

Electric field-induced concentration has the potential for application in highly sensitive detection of nanoparticles (NPs) for disease diagnosis and drug discovery. Conventional two-dimensional planar electrodes, however, have shown limited sensitivity in NP concentration. In this paper, the dielectrophoretic (DEP) concentration of low-abundance NPs is studied using a nanostructured tip where a high electric field of 3×10 ~7Vm ~1 is generated. In experimental studies, individual 2, 10, and 100nm Au NPs are concentrated to a nanotip using DEP concentration and are detected by scanning transmission and scanning electron microscopes. The DEP force on Au NPs near the end of a nanotip is computed according to the distance, and then compared with Brownian motion-induced force. The computational study shows qualitative agreement with the experimental results. When the experimental conditions for DEP concentration are optimized for 8nm-long oligonucleotides, the sensitivity of a nanotip is 10aM (10 attomolar; nine copies in a 1.5μl sample volume). This DEP concentrator using a nanotip can be used for molecular detection without amplification.
机译：电场引起的浓度具有潜力用于疾病诊断和药物发现的纳米颗粒（NPs）的高灵敏度检测。然而，常规的二维平面电极在NP浓度中显示出有限的灵敏度。本文使用纳米结构的尖端研究了低丰度NPs的介电泳（DEP）浓度，该尖端会产生3×10〜7Vm〜1的高电场。在实验研究中，使用DEP浓度将单个2、10和100nm的Au NP浓缩至纳米尖端，并通过扫描透射和扫描电子显微镜进行检测。根据距离计算纳米尖端末端附近金纳米颗粒上的DEP力，然后将其与布朗运动引起的力进行比较。计算研究表明，该结果与实验结果吻合良好。当针对8nm长的寡核苷酸优化DEP浓度的实验条件时，nanotip的灵敏度为10aM（10 atmol，在1.5μl样品体积中为9个拷贝）。这种使用纳米尖端的DEP浓缩器无需扩增即可用于分子检测。

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