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首页> 外文期刊>Nanotechnology >Optotransfection of mammalian cells based on a femtosecond laser and facilitated by gold nanorods
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Optotransfection of mammalian cells based on a femtosecond laser and facilitated by gold nanorods

机译：基于飞秒激光并通过金纳米棒促进的哺乳动物细胞的光转染

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摘要

The optotransfection of cells based on a femtosecond laser has attracted much attention owing to its high transfection efficiency and high cell viability since its first report by Konig. However, the low throughput in the original method also limits its use in practical applications. Gold nanoparticles (GNPs) have been reported to function as local receivers of light to relax the requirement of accurate optical alignment for the optotransfection of single cells. However, the visible light used in such work is not suitable for penetrating deep tissues in certain applications. In this study, we employed gold nanorods (GNRs) and an infrared femtosecond laser at the wavelength of 980 nm to realize optotransfection of cells with GFP. It was found that the surface coating of GNRs exhibited a significant effect on the process of cell permeabilization.
机译：基于飞秒激光的细胞光转染自从Konig的首次报告以来，由于其高转染效率和高细胞活力而备受关注。但是，原始方法的低通量也限制了其在实际应用中的使用。据报道，金纳米颗粒（GNP）可以作为光的局部接收器，从而放宽对单细胞进行光转染的精确光学对准的要求。但是，这种工作中使用的可见光不适合在某些应用中穿透深层组织。在这项研究中，我们采用了金纳米棒（GNR）和波长为980 nm的红外飞秒激光，以实现GFP细胞的光转染。发现GNR的表面涂层对细胞通透过程显示出显着影响。

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