• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nanotechnology >An impedimetric study of DNA hybridization on paper-supported inkjet-printed gold electrodes
【24h】

An impedimetric study of DNA hybridization on paper-supported inkjet-printed gold electrodes

机译：纸支撑喷墨印刷金电极上DNA杂交的阻抗研究

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this study, two different supramolecular recognition architectures for impedimetric detection of DNA hybridization have been formed on disposable papersupported inkjetprinted gold electrodes. The gold electrodes were fabricated using a gold nanoparticle based ink. The first recognition architecture consists of subsequent layers of biotinylated selfassembly monolayer (SAM), streptavidin and biotinylated DNA probe. The other recognition architecture is constructed by immobilization of thiolfunctionalized DNA probe (HSDNA) and subsequent backfill with 11mercapto1undecanol (MUOH) SAM. The binding capacity and selectivity of the recognition architectures were examined by surface plasmon resonance (SPR) measurements. SPR results showed that the HSDNA/ MUOH system had a higher binding capacity for the complementary DNA target. Electrochemical impedance spectroscopy (EIS) measurements showed that the hybridization can be detected with impedimetric spectroscopy in picomol range for both systems. EIS signal indicated a good selectivity for both recognition architectures, whereas SPR showed very high unspecific binding for the HSDNA/ MUOH system. The factors affecting the impedance signal were interpreted in terms of the complexity of the supramolecular architecture. The more complex architecture acts as a less ideal capacitive sensor and the impedance signal is dominated by the resistive elements. Keywords: electrochemical impedance spectroscopy, printed electronics, paper substrate,DNA, hybridization, surface plasmon resonance
机译：在这项研究中，在一次性纸质喷墨印刷金电极上形成了两种不同的超分子识别体系，用于DNA杂交的阻抗检测。金电极是使用基于金纳米粒子的油墨制造的。第一个识别结构由生物素化的自组装单层（SAM），链霉亲和素和生物素化的DNA探针的后续层组成。通过固定巯基官能化的DNA探针（HSDNA）并随后用11巯基十一烷醇（MUOH）SAM回填来构建其他识别结构。通过表面等离振子共振（SPR）测量来检查识别结构的结合能力和选择性。 SPR结果表明，HSDNA / MUOH系统对互补DNA靶标具有更高的结合能力。电化学阻抗谱（EIS）测量表明，对于两个系统，都可以在皮摩尔范围内用阻抗谱检测杂交。 EIS信号表明对两种识别结构都有良好的选择性，而SPR对HSDNA / MUOH系统显示出很高的非特异性结合。根据超分子结构的复杂性来解释影响阻抗信号的因素。较复杂的体系结构用作不太理想的电容传感器，并且阻抗信号由电阻元件主导。关键词：电化学阻抗谱，印刷电子，纸张基材，DNA，杂交，表面等离振子共振

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号