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Biocompatible fluorescent nanoparticles for in vivo stem cell tracking

机译：生物相容性荧光纳米颗粒用于体内干细胞追踪

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摘要

Efficient application of stem cells to the treatment of neurodegenerative diseases requires safe cell tracking to follow stem cell fate over time in the host environment after transplantation. In this work, for the first time, fluorescent and biocompatible methyl methacrylate (MMA)-based nanoparticles (fluoNPs) were synthesized through a free-radical co-polymerization process with a fluorescent macromonomer obtained by linking Rhodamine B and hydroxyethyl methacrylate. We demonstrate that the fluoNPs produced by polymerization of MMA-Rhodamine complexes (1) were efficient for the labeling and tracking of multipotent human amniotic fluid cells (hAFCs); (2) did not alter the main biological features of hAFCs (such as viability, cell growth and metabolic activity); (3) enabled us to determine the longitudinal bio-distribution of hAFCs in different brain areas after graft in the brain ventricles of healthy mice by a direct fluorescence-based technique. The reliability of our approach was furthermore confirmed by magnetic resonance imaging analyses, carried out by incubating hAFCs with both superparamagnetic iron oxide nanoparticles and fluoNPs. Our data suggest that these finely tunable and biocompatible fluoNPs can be exploited for the longitudinal tracking of stem cells.
机译：干细胞在神经退行性疾病治疗中的有效应用要求安全的细胞追踪，以便随时间推移在宿主环境中随时间推移跟踪干细胞的命运。在这项工作中，首次通过自由基共聚过程与通过将罗丹明B和甲基丙烯酸羟乙酯连接而获得的荧光大分子单体，通过荧光和生物相容性的甲基丙烯酸甲酯（MMA）基纳米粒子（fluoNPs）合成。我们证明，由MMA-若丹明复合物聚合产生的fluoNPs（1）对于标记和跟踪多能人羊水细胞（hAFC）是有效的; （2）不会改变hAFC的主要生物学特性（例如生存力，细胞生长和代谢活性）; （3）使我们能够通过基于荧光的直接技术确定健康小鼠的脑室中移植后hAFC在不同脑区的纵向生物分布。此外，通过将hAFC与超顺磁性氧化铁纳米粒子和fluoNP一起孵育，我们的磁共振成像分析进一步证实了我们方法的可靠性。我们的数据表明，这些可微调和生物相容的fluoNPs可用于干细胞的纵向追踪。

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