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Synthesis of water-dispersible zinc oxide quantum dots with antibacterial activity and low cytotoxicity for cell labeling

机译：具有抗菌活性和低细胞毒性的水分散性氧化锌量子点的合成，用于细胞标记

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摘要

Typical photoluminescent semiconductor nanoparticles, called quantum dots (QDs), have potential applications in biological labeling. When used to label stem cells, QDs may impair the differentiation capacity of the stem cells. In this study, we synthesized zinc oxide (ZnO) QDs in methanol with an average size of ～2 nm. We then employed two different types of polyethylene glycol (PEG) molecules (SH-PEG-NH_2 and NH_2-PEG-NH_2) to conjugate ZnO QDs and made them water-dispersible. Fourier transform infrared spectroscopy spectra indicated the attachment of PEG molecules on ZnO QDs. No obvious size alteration was observed for ZnO QDs after PEG conjugation. The water-dispersible ZnO QDs still retained the antibacterial activity and fluorescence intensity. The cytotoxicity evaluation revealed that ZnO QDs at higher concentrations decreased cell viability but were generally safe at 30 ppm or below. Cell lines of hepatocytes (HepG2), osteoblasts (MC3T3-E1) and mesenchymal stem cells (MSCs) were successfully labeled by the water-dispersible ZnO QDs at 30 ppm. The ZnO QD-labeled MSCs maintained their stemness and differentiation capacity. Therefore, we conclude that the water-dispersible ZnO QDs developed in this study have antibacterial activity, low cytotoxicity, and proper labeling efficiency, and can be used to label a variety of cells including stem cells.
机译：典型的光致发光半导体纳米粒子，称为量子点（QD），在生物标记中具有潜在的应用。当用于标记干细胞时，量子点可能会损害干细胞的分化能力。在这项研究中，我们在甲醇中合成了平均尺寸约为2 nm的氧化锌（ZnO）QD。然后，我们使用两种不同类型的聚乙二醇（PEG）分子（SH-PEG-NH_2和NH_2-PEG-NH_2）来共轭ZnO QD，并使它们水分散。傅里叶变换红外光谱表明，PEG分子附着在ZnO量子点上。 PEG结合后，ZnO量子点没有观察到明显的尺寸变化。水分散性ZnO QD仍保留了抗菌活性和荧光强度。细胞毒性评估表明，较高浓度的ZnO QD降低了细胞活力，但在30 ppm或更低的浓度下通常是安全的。肝细胞（HepG2），成骨细胞（MC3T3-E1）和间充质干细胞（MSCs）的细胞系已成功通过30 ppm水分散性ZnO QD进行标记。 ZnO QD标记的MSC保持其干性和分化能力。因此，我们得出的结论是，本研究开发的水分散性ZnO量子点具有抗菌活性，低细胞毒性和适当的标记效率，可用于标记多种细胞，包括干细胞。

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