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Synthesis of multifunctional large pore mesoporous silica nanoparticles as gene carriers

机译：多功能大孔介孔二氧化硅纳米粒子作为基因载体的合成

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摘要

The development of functional nanocarriers that can enhance the cellular delivery of a variety of nucleic acid agents is important in many biomedical applications such as siRNA therapy. We report the synthesis of large pore mesoporous silica nanoparticles (LPMSN) loaded with iron oxide and covalently modified by polyethyleneimine (denoted PEI-Fe-LPMSN) as carriers for gene delivery. The LPMSN have a particle size of ~200 nm and a large pore size of 11 nm. The large pore size is essential for the formation of large iron oxide nanoparticles to increase the magnetic properties and the adsorption capacity of siRNA molecules. The magnetic property facilitates the cellular uptake of nanocarriers under an external magnetic field. PEI is covalently grafted on the silica surface to enhance the nanocarriers' affinity against siRNA molecules and to improve gene silencing performance. The PEI-Fe-LPMSN delivered siRNA-PLK1 effectively into osteosarcoma cancer cells, leading to cell viability inhibition of 80%, higher compared to the 50% reduction when the same dose of siRNA was delivered by a commercial product, oligofectamine.
机译：在许多生物医学应用（例如siRNA治疗）中，可以增强多种核酸试剂在细胞中递送的功能性纳米载体的开发非常重要。我们报告了大孔介孔二氧化硅纳米粒子（LPMSN）的合成与氧化铁负载和共价修饰的聚乙烯亚胺（表示为PEI-Fe-LPMSN）作为载体的基因传递。 LPMSN的粒径约为200 nm，大孔径约为11 nm。大孔径对于形成大的氧化铁纳米颗粒以增加磁性和siRNA分子的吸附能力至关重要。磁性有助于在外部磁场下细胞对纳米载体的吸收。 PEI共价接枝在二氧化硅表面上，以增强纳米载体对siRNA分子的亲和力并改善基因沉默性能。 PEI-Fe-LPMSN有效地将siRNA-PLK1传递到骨肉瘤癌细胞中，导致细胞活力抑制为80％，高于通过商业产品oligofectamine传递相同剂量的siRNA时的50％降低。

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