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In vivo monitoring of distributional transport kinetics and extravasation of quantum dots in living rat liver

机译:活体内大鼠肝脏中分布转运动力学和量子点外渗的体内监测

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摘要

Although the unique optical properties of surface-modified quantum dots (QDs) have attracted wide interest in molecular biology and bioengineering, there are very few reports of their in vivo biodistribution, due to a lack of analytical techniques for characterizing the dynamic variation of QDs in living animals. In this study, we used an in vivo online monitoring system and a batch-wise elemental analytical method to investigate the biodistribution/ extravasation of various surface-modified CdTeSe/ZnS (QDs) in rat liver. It is found that the surface modification dictated not only the blood retention profile but also the degree of extravasation and the clearance of extracellular QDs, making it an important variable for regulating the transfer and exchange process of QDs among three physiological compartments - bloodstream, extracellular space and Kupffer cells/hepatocytes.
机译:尽管表面修饰量子点(QD)的独特光学性质已引起分子生物学和生物工程学的广泛兴趣,但由于缺乏表征QDs动态变化的分析技术,有关其体内生物分布的报道很少。活体动物。在这项研究中,我们使用了体内在线监测系统和分批元素分析方法来研究大鼠肝脏中各种表面修饰的CdTeSe / ZnS(QD)的生物分布/外渗。结果发现,表面修饰不仅决定了血液的滞留状况,而且决定了渗出的程度和细胞外QD的清除,使其成为调节QD在三个生理区室(血流,细胞外空间)之间转移和交换过程的重要变量。和枯否细胞/肝细胞。

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