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首页> 外文期刊>Nanotechnology >Fabrication of birefringent nanocylinders for single-molecule force and torque measurement
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Fabrication of birefringent nanocylinders for single-molecule force and torque measurement

机译：制备用于单分子力和扭矩的双折射纳米圆柱体

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Optically anisotropic subwavelength scale dielectric particles have been shown to enable studies of the mechanical properties of bio-molecules via optical trapping and manipulation. However, techniques emphasized to date for fabrication of such particles generally suffer from limited uniformity and control over particle dimensions, or low throughput and high cost. Here, an approach for rapid, low-cost, fabrication of large quantities of birefringent quartz nanocylinders with dimensions optimized for optical torque wrench experiments is described. For a typical process, 10~8 or more quartz cylinders with diameters of 500 nm and heights of 800 nm, with uniformity of ±5% in each dimension, can be fabricated over ~10 cm~2 areas, for binding to a single bio-molecule, and harvested for use in optical trapping experiments. Use of these structures to measure extensional and torsional dynamics of single DNA molecules is demonstrated with measured forces and torques shown to be in very good agreement with previously reported results.
机译：光学各向异性亚波长尺度介电粒子已被证明能够通过光学捕获和操纵研究生物分子的机械性能。然而，迄今为止强调的用于制造这种颗粒的技术通常遭受有限的均匀性和对颗粒尺寸的控制，或者产量低且成本高。在此，描述了一种快速，低成本，大量制造具有针对光学扭矩扳手实验而优化尺寸的双折射石英纳米圆筒的方法。对于一个典型的过程，可以在〜10 cm〜2的面积上制造10〜8个或更多直径为500 nm，高度为800 nm，均匀度为±5％的石英圆柱，以与单个生物体结合-分子，并收集用于光阱实验。这些结构用于测量单个DNA分子的拉伸和扭转动力学，证明了所测得的力和扭矩与先前报道的结果非常吻合。

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