• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nanotechnology >Dual-colored graphene quantum dotslabeled nanoprobes/graphene oxide: functional carbon materials for respective and simultaneous detection of DNA and thrombin
【24h】

Dual-colored graphene quantum dotslabeled nanoprobes/graphene oxide: functional carbon materials for respective and simultaneous detection of DNA and thrombin

机译：标记为纳米探针/氧化石墨烯的双色石墨烯量子点：分别用于同时检测DNA和凝血酶的功能碳材料

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Convenient and simultaneous detection of multiple biomarkers such as DNA and proteins with biocompatible materials and good analytical performance still remains a challenge. Herein, we report the respective and simultaneous detection of DNA and bovine α-thrombin (thrombin) entirely based on biocompatible carbon materials through a specially designed fluorescence onoff-on process. Colorful fluorescence, high emission efficiency, good photostability and excellent compatibility enables graphene quantum dots (GQDs) as the best choice for fluorophores in bioprobes, and thus two-colored GQDs as labeling fluorophores were chemically bonded with specific oligonucleotide sequence and aptamer to prepare two probes targeting the DNA and thrombin, respectively. Each probe can be assembled on the graphene oxide (GO) platform spontaneously by π-π stacking and electrostatic attraction; as a result, fast electron transfer in the assembly efficiently quenches the fluorescence of probe. The presence of DNA or thrombin can trigger the self-recognition between capturing a nucleotide sequence and its target DNA or between thrombin and its aptamer due to their specific hybridization and duplex DNA structures or the formation of apatamer-substrate complex, which is taken advantage of in order to achieve a separate quantitative analysis of DNA and thrombin. A dual-functional biosensor for simultaneous detection of DNA and thrombin was also constructed by self-assembly of two probes with distinct colors and GO platform, and was further evaluated with the presence of various concentrations of DNA and thrombin. Both biosensors serving as a general detection model for multiple species exhibit outstanding analytical performance, and are expected to be applied in vivo because of the excellent biocompatibility of their used materials.
机译：利用生物相容性材料方便，同时地检测多种生物标记物（例如DNA和蛋白质）以及良好的分析性能仍然是一个挑战。在此，我们报告了通过专门设计的荧光开关过程，完全基于生物相容性碳材料分别同时检测DNA和牛α-凝血酶（凝血酶）。彩色荧光，高发射效率，良好的光稳定性和出色的相容性使石墨烯量子点（GQD）成为生物探针中荧光团的最佳选择，因此将两种颜色的GQD作为标记荧光团与特定的寡核苷酸序列和适体化学键合，从而制备了两个探针分别靶向DNA和凝血酶。每个探针都可以通过π-π堆积和静电吸引而自发地组装在氧化石墨烯（GO）平台上。结果，组件中的快速电子转移有效地淬灭了探针的荧光。 DNA或凝血酶的存在可触发捕获核苷酸序列与其靶DNA之间或凝血酶与其适体之间的自我识别，这是由于它们的特异性杂交和双链DNA结构或形成了适体-底物复合物所致，为了实现对DNA和凝血酶的单独定量分析。通过两种颜色和GO平台不同的探针的自组装，构建了一种同时检测DNA和凝血酶的双功能生物传感器，并用各种浓度的DNA和凝血酶进行了进一步评估。两种生物传感器都可以作为多种物种的通用检测模型，它们均具有出色的分析性能，并且由于其使用的材料具有出色的生物相容性，因此有望在体内应用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号